Evidence map›Paper›PMID 32755529›Full record

ArticleJournal of medical microbiology2020

Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA.

Jean Y H Lee, Nickala Best, Julie McAuley, Jessica L Porter, Torsten Seemann, Mark B Schultz, Michelle Sait, Nicole Orlando, Karolina Mercoulia, Susan A Ballard and 18 more

Abstract readValidation Study
In one paragraph

Article in Journal of medical microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed, 6 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 6 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
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  5. Pooled it
  6. Pooled it
  7. Review
  8. Review
  9. Biology methods & protocols · 2026
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  12. Article
  13. A low-cost and versatile paramagnetic bead DNA extraction method forApplied and environmental microbiology · 2024
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  14. Article
  15. Article
  16. Improved protocol forExperimental biology and medicine (Maywood, N.J.) · 2023
    Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Jean Y H LeeDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Nickala BestGenWorks Pty Ltd, Thebarton, South Australia, Australia.
Julie McAuleyDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Jessica L PorterDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Torsten SeemannDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Mark B SchultzMicrobiological Diagnostic Unit Public Health Laboratory, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Michelle SaitMicrobiological Diagnostic Unit Public Health Laboratory, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Nicole OrlandoMicrobiological Diagnostic Unit Public Health Laboratory, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Karolina MercouliaMicrobiological Diagnostic Unit Public Health Laboratory, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Susan A BallardMicrobiological Diagnostic Unit Public Health Laboratory, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Julian DruceVictorian Infectious Diseases Reference Laboratory, Melbourne Health at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Thomas TranVictorian Infectious Diseases Reference Laboratory, Melbourne Health at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Mike G CattonVictorian Infectious Diseases Reference Laboratory, Melbourne Health at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Melinda J Pryor360Biolabs, Melbourne, Victoria, Australia.
Huanhuan L Cui360Biolabs, Melbourne, Victoria, Australia.
Angela Luttick360Biolabs, Melbourne, Victoria, Australia.
Sean McDonaldGenWorks Pty Ltd, Thebarton, South Australia, Australia.
Arran GreenhalghGenWorks Pty Ltd, Thebarton, South Australia, Australia.
Jason C KwongDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Norelle L SherryMicrobiological Diagnostic Unit Public Health Laboratory, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Maryza GrahamDepartment of Microbiology, Monash Health, Clayton, Victoria, Australia.
Tuyet HoangDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Marion HerisseDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Sacha J PidotDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Deborah A WilliamsonDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Benjamin P HowdenDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Ian R MonkDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Timothy P StinearDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PubMed holds no abstract for this paper.

Indexed as

BetacoronavirusClinical Laboratory TechniquesCoronavirus InfectionsCOVID-19COVID-19 TestingCOVID-19 VaccinesHumansMolecular Diagnostic TechniquesNasopharynxNucleic Acid Amplification TechniquesPandemicsPneumonia, ViralReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionReverse TranscriptionRNA, ViralCovid-19 aAPC vaccineCOVID-19 VaccinesRNA, Viralnasopharyngeal swabsRT-LAMPSARS-CoV-2universal transport media

Identifiers

PMID32755529
PMCPMC7656183

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.