Evidence map›Paper›PMID 32754855›Full record

ArticleIn vitro cellular & developmental biology. Animal2020

Cyanidin improves oocyte maturation and the in vitro production of pig embryos.

Emma Hicks, Miranda Mentler, Haley A Arena, Jaelyn Z Current, Brian D Whitaker

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Emma HicksDepartment of Animal and Pre-veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA.
Miranda MentlerDepartment of Animal and Pre-veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA.
Haley A ArenaDepartment of Animal and Pre-veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA.
Jaelyn Z CurrentDepartment of Animal and Pre-veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA.
Brian D WhitakerDepartment of Animal and Pre-veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA. whitaker@findlay.edu.ORCID http://orcid.org/0000-0001-8032-0845
University of Findlay · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objective of this study was to reduce the negative effects of oxidative stress by decreasing the levels of reactive oxygen species (ROS) through supplementation of the major antioxidants present in elderberries: kuromanin and cyanidin. Oocytes (n = 1150) were supplemented with 100 or 200 μM of kuromanin or cyanidin during maturation, and then evaluated for ROS levels or fertilized and evaluated for penetration, polyspermic penetration, male pronucleus formation, and embryonic development. The ROS levels and incidence of polyspermic penetration were lower (P < 0.05) in oocytes supplemented with 100 μM cyanidin when compared with other treatments. Supplementation of 100 μM cyanidin increased (P < 0.05) MPN and blastocyst formation compared with other treatments. However, supplementation of 100 μM kuromanin did not have significant effects on the criteria evaluated, and supplementation of 200 μM kuromanin had significant (P < 0.05) detrimental effects for each criterion. Additional oocytes (n = 1438) were supplemented with 100 μM cyanidin during maturation and evaluated for glutathione, glutathione peroxidase, catalase, and superoxide dismutase activity. Supplementation of 100 μM cyanidin increased (P < 0.05) catalase activity and intracellular GSH levels compared with no supplementation of cyanidin. These results indicate that supplementing cyanidin during maturation reduces oxidative stress by reducing ROS levels and increasing GSH concentrations within the oocyte.

Indexed as

Embryo Culture TechniquesIn Vitro Oocyte Maturation TechniquesAnimalsAnthocyaninsEmbryo, MammalianEmbryonic DevelopmentFertilization in VitroGlucosidesModels, BiologicalOocytesReactive Oxygen SpeciesSwineAnthocyaninscyanidincyanidin 3-O-glucopyranosideGlucosidesReactive Oxygen SpeciesCyanidinElderberryKuromaninOocytePorcineROS

Identifiers

PMID32754855
OpenAlexW3047558249

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.