Evidence map›Paper›PMID 32754814›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Avians as a Model System of Vascular Development.

Rieko Asai, Michael Bressan, Takashi Mikawa

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Differential sensitivity of midline development to mitosis during and after primitive streak extension.Developmental dynamics : an official publication of the American Association of Anatomists · 2026
    Article
  3. The avian embryo as a time-honoured animal model in developmental, biomedical and agricultural research.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  4. Bilateral cellular flows display asymmetry prior to left-right organizer formation in amniote gastrulation.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Rieko AsaiCardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA.
Michael BressanDepartment of Cell Biology and Physiology, McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Takashi MikawaCardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA. takashi.mikawa@ucsf.edu.

Funding

Regulation of cardiac pacemaker cell cytoarchitectureR01HL146626 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Michael C Bressan · 2019 to 2026
$3.9M
Coronary Vascular Cell DevelopmentR37HL078921 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MIKAWA, TAKASHI · 2010 to 2019
$3.8M
Cardiac Pacemaker DevelopmentR01HL112268 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MIKAWA, TAKASHI · 2012 to 2016
$1.9M
Induction and Patterning of Cardiogenic FieldsR01HL153736 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MIKAWA, TAKASHI · 2020 to 2023
$1.6M
Development of Vascular Smooth Muscle Stem Cell NicheR01HL148125 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MIKAWA, TAKASHI · 2019 to 2022
$1.6M
Atrioventricular Junction DevelopmentR01HL132832 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MIKAWA, TAKASHI · 2016 to 2019
$1.6M
Heart Field DevelopmentR01HL122375 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MIKAWA, TAKASHI · 2014 to 2017
$1.6M
Developmental Patterning of the Sinoatrial NodeR00HL122360 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI BRESSAN, MICHAEL C · 2016 to 2018
$745k
NHLBI NIH HHS R00 HL122360NHLBI NIH HHS R01 HL112268NHLBI NIH HHS R01 HL122375NHLBI NIH HHS R01 HL132832NHLBI NIH HHS R01 HL146626NHLBI NIH HHS R01 HL148125NHLBI NIH HHS R01 HL153736NHLBI NIH HHS R37 HL078921
6 · The paper itself

Abstract

For more than 2000 years, the avian embryo has helped scientists understand questions of developmental and cell biology. As early as 350 BC Aristotle described embryonic development inside a chicken egg (Aristotle, Generation of animals. Loeb Classical Library (translated), vol. 8, 1943). In the seventeenth century, Marcello Malpighi, referred to as the father of embryology, first diagramed the microscopic morphogenesis of the chick embryo, including extensive characterization of the cardiovascular system (Pearce Eur Neurol 58(4):253-255, 2007; West, Am J Physiol Lung Cell Mol Physiol 304(6):L383-L390, 2016). The ease of accessibility to the embryo and similarity to mammalian development have made avians a powerful system among model organisms. Currently, a unique combination of classical and modern techniques is employed for investigation of the vascular system in the avian embryo. Here, we will introduce the essential techniques of embryonic manipulation for experimental study in vascular biology.

Indexed as

AnimalsChick EmbryoChickensEmbryo, MammalianEmbryonic DevelopmentModels, AnimalNeovascularization, PhysiologicQuailAvian systemCell labelingElectroporationSomatic transgenesisTransductionVascular development

Identifiers

PMID32754814
PMCPMC7537975

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.