ArticleFrontiers in genetics2020
Identification of Gene Modules and Hub Genes Involved in Mastitis Development Using a Systems Biology Approach.
Article in Frontiers in genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 53 citations in OpenAlex.
- Multi-Layer Transcriptome Analysis Uncovers Molecular Signatures of Coliform Mastitis in Lactating Cows.International journal of molecular sciences · 2026Article
- Article
- RNA stability: a novel perspective on gene regulatory networks in bovine mastitis.BMC genomics · 2025Article
- Single-step genome-wide association study of milk somatic cell scores across multi-cattle breeds in Ethiopia.Animal biotechnology · 2025Article
- Identification of Genomic Structural Variations in Xinjiang Brown Cattle by Deep Sequencing and Their Association with Body Conformation Traits.International journal of molecular sciences · 2025Article
- Unlocking the genetic code: a comprehensive Genome-Wide association study and gene set enrichment analysis of cell-mediated immunity in chickens.BMC genomics · 2025Article
- TNFRSF1A and NCF1 May Act as Hub Genes in Mastitis.Veterinary medicine and science · 2025Article
- Integrative Multiomics in the Lung Reveals a Protective Role of Asporin in Pulmonary Arterial Hypertension.Circulation · 2024Article
- Identification of hub genes and their expression profiling for predicting buffalo (Bubalus bubalis) semen quality and fertility.Scientific reports · 2023Article
- Identification and Classification of Long Non-Coding RNAs in the Mammary Gland of the Holstein Cow.International journal of molecular sciences · 2023Article
- Genomic Characterization and Initial Insight into Mastitis-Associated SNP Profiles of Local LatvianAnimals : an open access journal from MDPI · 2023Article
- Integrated analysis of inflammatory mRNAs, miRNAs, and lncRNAs elucidates the molecular interactome behind bovine mastitis.Scientific reports · 2023Article
- Longitudinal APOE4- and amyloid-dependent changes in the blood transcriptome in cognitively intact older adults.Alzheimer's research & therapy · 2023Article
- Punch-excised explants of bovine mammary gland to model early immune response to infection.Journal of animal science and biotechnology · 2023Article
- Transcriptome-wide mapping of milk somatic cells upon subclinical mastitis infection in dairy cattle.Journal of animal science and biotechnology · 2023Article
- Article
- Candidate genes for mastitis resistance in dairy cattle: a data integration approach.Journal of animal science and biotechnology · 2023Article
- The central inflammatory regulator IκBζ: induction, regulation and physiological functions.Frontiers in immunology · 2023Review
- Transcriptome Analysis of Goat Mammary Gland Tissue Reveals the Adaptive Strategies and Molecular Mechanisms of Lactation and Involution.International journal of molecular sciences · 2022Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveMastitis is defined as the inflammation of the mammary gland, which impact directly on the production performance and welfare of dairy cattle. Since, mastitis is a multifactorial complex disease and the molecular pathways underlying this disorder have not been clearly understood yet, a system biology approach was used in this study to a better understanding of the molecular mechanisms behind mastitis.
methodsPublicly available RNA-Seq data containing samples from milk of five infected and five healthy Holstein cows at five time points were retrieved. Gene Co-expression network analysis (WGCNA) approach and functional enrichment analysis were then applied with the aim to find the non-preserved module of genes that their connectivity were altered under infected condition. Hub genes were identified in the non-preserved modules and were subjected to protein-protein interactions (PPI) network construction.
resultsAmong the 25 modules identified, eight modules were non-preserved and were also biologically associated with inflammation, immune response and mastitis development. Interestingly most of the hub genes in the eight modules were also densely connected in the PPI network. Of the hub genes, 250 genes were hubs in both co-expression and PPI networks and most of them were reported to play important roles in immune response or inflammatory pathways. The blue module was highly enriched in inflammatory responses and
conclusionThis study improved the understanding of the mechanisms underlying bovine mastitis and suggested eight non-preserved modules along with several most important genes with promising potential in etiology of mastitis.
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