Evidence map›Paper›PMID 32749923›Full record

ArticleNucleic acid therapeutics2021

Modulating Polymer-siRNA Binding Does Not Promote Polyplex-Mediated Silencing.

R Chauncey Splichal, Joseph A Gredell, Erin B Vogel, Amanda Malefyt, Georgina Comiskey, Milton R Smith, Christina Chan, S Patrick Walton

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acid therapeutics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

R Chauncey SplichalDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, USA.
Joseph A GredellDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, USA.
Erin B VogelDepartment of Chemistry, and Michigan State University, East Lansing, Michigan, USA.
Amanda MalefytDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, USA.
Georgina ComiskeyDepartment of Chemistry, and Michigan State University, East Lansing, Michigan, USA.
Milton R SmithDepartment of Chemistry, and Michigan State University, East Lansing, Michigan, USA.
Christina ChanDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, USA.
S Patrick WaltonDepartment of Chemical Engineering and Materials Science, Michigan State University, East Lansing, Michigan, USA.
Michigan State University · USDow Chemical (United States) · USHowell Laboratories (United States) · USNovozymes (United States) · USTrine University · US

Funding

Integrative Training in the Pharmacological SciencesT32GM092715 · NIGMS · MICHIGAN STATE UNIVERSITY · PI NEUBIG, RICHARD R · 2011 to 2020
$1.5M
NIGMS NIH HHS T32 GM092715
6 · The paper itself

Abstract

The development of delivery vehicles for small interfering RNAs (siRNAs) remains a bottleneck to widespread clinical use. Cationic polymers represent an important class of potential delivery vehicles. In this study, we used alkyne-azide click chemistry to synthesize a variety of cationic poly(propargyl glycolide) backbone polymers to bind and deliver siRNAs. We demonstrated control over the binding interactions of these polymers and siRNAs by varying binding strength by more than three orders of magnitude. Binding strength was found to meet or exceed that of commercially available transfection agents. Our polymers effectively delivered siRNAs with no detectable cytotoxicity. Despite accumulation of siRNAs at levels comparable with commercial reagents, we did not observe silencing of the targeted protein. The implications of our results for future siRNA delivery vehicle design are discussed.

Indexed as

PolymersCationsRNA, Small InterferingTransfectionCationsPolymersRNA, Small Interferingcationic polymerspoly(propargyl glycolide)siRNA delivery

Identifiers

PMID32749923
PMCPMC8215420
OpenAlexW3047426090

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.