Evidence map›Paper›PMID 32748696›Full record

ArticlemAbs

The Antibody Society's antibody validation webinar series.

Jan L A Voskuil, Anita Bandrowski, C Glenn Begley, Andrew R M Bradbury, Andrew D Chalmers, Aldrin V Gomes, Travis Hardcastle, Fridtjof Lund-Johansen, Andreas Plückthun, Giovanna Roncador and 5 more

Abstract read
In one paragraph

Article in mAbs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
  2. Article
  3. SUMO Antibody Validation.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Construction of Synthetic Antibody Phage Display Libraries.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Antibody Validation for Estrogen Receptor Beta.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  17. Article
  18. Article
  19. Article
  20. BRCA1 Antibodies Matter.International journal of biological sciences · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jan L A VoskuilAeonian Biotech Ltd , Marcham, UK.ORCID 0000-0003-0799-0548
Anita BandrowskiCenter for Research in Biological Systems, University of California , La Jolla, CA, USA.ORCID 0000-0002-5497-0243
C Glenn BegleyBioCurate , Parkville, Australia.ORCID 0000-0003-2419-6215
Andrew R M BradburySpecifica Inc ., Santa Fe, NM, USA.ORCID 0000-0002-5567-8172
Andrew D ChalmersDepartment of Biology and Biochemistry, University of Bath , Bath, UK.ORCID 0000-0003-4182-6717
Aldrin V GomesDepartment of Neurobiology, Physiology, and Behavior, University of California Davis , Davis, CA, USA.ORCID 0000-0002-9819-3036
Travis HardcastleHorizon Discovery , Waterbeach, Cambridge, UK.
Fridtjof Lund-JohansenDepartment of Immunology, Oslo University Hospital , Oslo, Norway.ORCID 0000-0002-2445-1258
Andreas PlückthunDepartment. of Biochemistry, University of Zurich , Zurich, Switzerland.ORCID 0000-0003-4191-5306
Giovanna RoncadorMonoclonal Antibody Unit, Spanish National Cancer Research Center , Madrid, Spain.ORCID 0000-0002-9807-2875
Alejandra SolacheAbcam Plc, Discovery Drive, Cambridge Biomedical Campus , Cambridge, UK.
Michael J TaussigCambridge Protein Arrays Ltd., Babraham Research Campus , Cambridge, UK.
James S TrimmerDepartment of Physiology and Membrane Biology, University of California Davis School of Medicine , Davis, CA, USA.ORCID 0000-0002-6117-3912
Cecilia WilliamsScience for Life Laboratory, Department of Protein Science, KTH Royal Institute of Technology , Solna, Sweden.ORCID 0000-0002-0602-2062
Simon L GoodmanThe Antibody Society, Inc ., Framingham, MA, USA.ORCID 0000-0002-3480-1346

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the wake of the reproducibility crisis and numerous discussions on how commercially available antibodies as research tool contribute to it, The Antibody Society developed a series of 10 webinars to address the issues involved. The webinars were delivered by speakers with both academic and commercial backgrounds. This report highlights the problems, and offers solutions to help the scientific community appropriately identify the right antibodies and to validate them for their research and development projects. Despite the various solutions proposed here, they must be applied on a case-by-case basis. Each antibody must be verified based on the content of the product sheet, and subsequently through experimentation to confirm integrity, specificity and selectivity. Verification needs to focus on the precise application and tissue/cell type for which the antibody will be used, and all verification data must be reported openly. The various approaches discussed here all have caveats, so a combination of solutions must be considered.

Indexed as

Antibodies, MonoclonalAntibody SpecificityProtein EngineeringValidation Studies as TopicHumansWebcasts as TopicAntibodies, Monoclonalantibody selectivityAntibody specificityantibody validationmonoclonal antibodiespeptide antibodiespolyclonal antibodiesrecombinant antibodiesreproducibilityselective antibodiesspecific antibodies

Identifiers

PMID32748696
PMCPMC7531563

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.