Evidence map›Paper›PMID 32748166›Full record

ArticleLasers in medical science2021

Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy.

Kumiko Koibuchi Sakane, Tanmoy Bhattacharjee, Jaciara Fagundes, Luciana Maria Cortez Marcolino, Isabelle Ferreira, Juliana Guerra Pinto, Juliana Ferreira-Strixino

Abstract read
PubMed Publisher
In one paragraph

Article in Lasers in medical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Vaterite microparticle-loaded methylene blue for photodynamic activity in macrophages infected with Leishmania braziliensis.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Kumiko Koibuchi SakaneInfrared Spectroscopy Laboratory, Research and Development Institute R&DI, University of Vale do Paraíba - Univap, Shishima Hifumi Avenue, 2911, São Jose dos Campos, São Paulo, 12244-000, Brazil.
Tanmoy BhattacharjeeSir John Walsh Research Institute, 310 Great King Street, Dunedin, 9016, New Zealand.
Jaciara FagundesPhotobiology Applied to Health Laboratory - Research and Development Institute - PI&D, University of Vale do Paraiba, Univap, Shishima Hifumi Avenue 2911, São José dos Campos, São Paulo, 12244-000, Brazil.
Luciana Maria Cortez MarcolinoPhotobiology Applied to Health Laboratory - Research and Development Institute - PI&D, University of Vale do Paraiba, Univap, Shishima Hifumi Avenue 2911, São José dos Campos, São Paulo, 12244-000, Brazil.
Isabelle FerreiraPhotobiology Applied to Health Laboratory - Research and Development Institute - PI&D, University of Vale do Paraiba, Univap, Shishima Hifumi Avenue 2911, São José dos Campos, São Paulo, 12244-000, Brazil.
Juliana Guerra PintoPhotobiology Applied to Health Laboratory - Research and Development Institute - PI&D, University of Vale do Paraiba, Univap, Shishima Hifumi Avenue 2911, São José dos Campos, São Paulo, 12244-000, Brazil.ORCID http://orcid.org/0000-0002-7356-1576
Juliana Ferreira-StrixinoPhotobiology Applied to Health Laboratory - Research and Development Institute - PI&D, University of Vale do Paraiba, Univap, Shishima Hifumi Avenue 2911, São José dos Campos, São Paulo, 12244-000, Brazil. jufestrixino@gmail.br.
Universidade do Vale do Paraíba · BR

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/12211-4
6 · The paper itself

Abstract

Photodynamic therapy (PDT) with photosensitizer methylene blue was applied to Leishmania braziliensis, and Fourier transform infrared (FTIR) spectroscopy was used to study biochemical changes in the parasite after PDT in comparison to untreated (C), only irradiation (I), and only photosensitizer (PS). Spectral analysis suggests increase in lipids, proteins, and protein secondary structures in PDT compared with C and decrease in nucleic acids and carbohydrates. Interestingly, these trends are different from PDT of Leishmania major species, wherein lipids decrease; there are minimal changes in secondary structures and increase in nucleic acids and carbohydrates. The study thus suggests possibility of different biomolecular players/pathways in PDT-induced death of L. braziliensis and L. major.

Indexed as

PhotochemotherapySpectroscopy, Fourier Transform InfraredLeishmania braziliensisMethylene BluePhotosensitizing AgentsProtozoan ProteinsMethylene BluePhotosensitizing AgentsProtozoan ProteinsFTIR spectroscopyLeishmania braziliensisMethylene bluePhotodynamic therapy

Identifiers

PMID32748166
OpenAlexW3046977269

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.