ArticleVeterinary research2020
Proteomic analysis of bovine mammary epithelial cells after in vitro incubation with S. agalactiae: potential biomarkers.
Article in Veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Maternal exercise during lactation remodels obesity-associated mammary metabolism and milk fatty acids, enhancing offspring lipid oxidation.American journal of physiology. Endocrinology and metabolism · 2026Article
- UID-Dual Transcriptome Sequencing Analysis of the Molecular Interactions betweenAnimals : an open access journal from MDPI · 2024Article
- Unveiling the power of proteomics in advancing tropical animal health and production.Tropical animal health and production · 2024Article
- Performance evaluation of a rapid immunochromatographic test kit in detecting bovine mastitis-causing streptococci.The Journal of veterinary medical science · 2024Article
- Lactococcus garvieae exerts a critical role in inducing inflammation in dairy mastitis by triggering NLRP3 inflammasome-mediated pyroptosis in MAC-T cells.World journal of microbiology & biotechnology · 2024Article
- Genotype Distribution and High-Risk Factors Analysis of Group B Streptococcus in Late-Stage Pregnant Women in the Linyi Region.International journal of microbiology · 2024Article
- Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells byiScience · 2023Article
- Metataxonomic analysis and host proteome response in dairy cows with high and low somatic cell count: a quarter level investigation.Veterinary research · 2023Article
- Effect of anemoside B4 on milk whey in clinical mastitis-affected cows elucidated using tandem mass tag (TMT)-based quantitative proteomics.Scientific reports · 2022Article
- The Analysis of the Ubiquitylomic Responses toJournal of inflammation research · 2022Article
- The Role ofMicroorganisms · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Streptococcus agalactiae is one of the causative agents of subclinical mastitis, a common disease of dairy cows that causes great economic losses in the industry worldwide. It is thought that pathology is mainly due to inflammatory damage of bovine mammary epithelial cells (bMECs); however, the mechanism by which S. agalactiae damages the bMECs is not clear. The aim of this study was to evaluate the inflammatory effects of S. agalactiae on bMECs and the resulting changes in protein profiles. The bMECs were incubated with S. agalactiae for different times and assayed for cell viability by MTT assay, apoptosis by annexin V and propidium iodide dual staining, and morphological and ultrastructural changes by scanning and transmission electron microscopy. Quantitative real-time PCR was used to determine the effect of S. agalactiae on expression of mRNA of inflammatory factors in bMECs and protein levels were quantitated by liquid chromatography/mass spectrometry. Exposure to S. agalactiae significantly decreased the cell viability and triggered apoptosis, as well as up-regulating TNF-α, IL-1β and IL-6 mRNA, and inhibiting IL-8 expression. S. agalactiae also induced morphological and ultrastructural changes. Furthermore, we identified 325 up-regulated and 704 down-regulated proteins in the treated vs control group. All significant differentially expressed proteins (DSEPs) were classified into three major areas by function: biological processes, cellular components and molecular functions. These differentially expressed proteins included enzymes and proteins associated with various metabolic processes and cellular immunity. Pathway enrichment analysis showed that eight down-regulated signaling pathways were significantly enriched. Exposure to even subclinical levels of S. agalactiae can lead to inflammation and bMEC damage. Our data suggest some possible molecular mechanisms for the harmful effects of subclinical mastitis in dairy cows.
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Registered trials
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