Evidence map›Paper›PMID 32745985›Full record

ArticleiScience2020

Spatiotemporal Gradient and Instability of Wnt Induce Heterogeneous Growth and Differentiation of Human Intestinal Organoids.

Woojung Shin, Alexander Wu, Soyoun Min, Yong Cheol Shin, R Y Declan Fleming, S Gail Eckhardt, Hyun Jung Kim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed, 1 pooled it
3.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.

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  14. A scalable long-termbioRxiv : the preprint server for biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Woojung ShinDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton St., Austin, TX 78712, USA.
Alexander WuDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton St., Austin, TX 78712, USA.
Soyoun MinDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton St., Austin, TX 78712, USA.
Yong Cheol ShinDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton St., Austin, TX 78712, USA.
R Y Declan FlemingDepartment of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX 78712, USA; Department of Surgery and Perioperative Care, Dell Medical School, The University of Texas at Austin, Austin, TX 78712, USA.
S Gail EckhardtDepartment of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX 78712, USA.
Hyun Jung KimDepartment of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton St., Austin, TX 78712, USA; Department of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX 78712, USA; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. Electronic address: hyunjung.kim@utexas.edu.
The University of Texas at Austin · US

Funding

A personalized colorectal cancer-on-a-chip for assessing tumor-microbiome crosstalkR21CA236690 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI KIM, HYUN JUNG · 2019 to 2021
$523k
Investigation of the Carcinogenic Effects of Bactericidal Antibiotics in the GutK00CA245801 · NCI · HARVARD UNIVERSITY · PI SHIN, WOOJUNG · 2021 to 2023
$278k
Deciphering the Role of Gut Microbiome in Colitis-Associated Colorectal Cancer Using a Patient-Specific Disease-on-a-ChipF99CA245801 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI SHIN, WOOJUNG · 2019 to 2020
$75k
NCI NIH HHS F99 CA245801NCI NIH HHS K00 CA245801NCI NIH HHS R21 CA236690
6 · The paper itself

Abstract

In a conventional culture of three-dimensional human intestinal organoids, extracellular matrix hydrogel has been used to provide a physical space for the growth and morphogenesis of organoids in the presence of exogenous morphogens such as Wnt3a. We found that organoids embedded in a dome-shaped hydrogel show significant size heterogeneity in different locations inside the hydrogel. Computational simulations revealed that the instability and diffusion limitation of Wnt3a constitutively generate a concentration gradient inside the hydrogel. The location-dependent heterogeneity of organoids in a hydrogel dome substantially perturbed the transcriptome profile associated with epithelial functions, cytodifferentiation including mucin 2 expression, and morphological characteristics. This heterogeneous phenotype was significantly mitigated when the Wnt3a was frequently replenished in the culture medium. Our finding suggests that the morphological, transcriptional, translational, and functional heterogeneity in conventional organoid cultures may lead to a false interpretation of the experimental results in organoid-based studies.

Indexed as

CancerComputer ModelingTissue Engineering

Identifiers

PMID32745985
PMCPMC7398973
OpenAlexW3042690990

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.