ArticleThe Biochemical journal2020
The composition and turnover of the Arabidopsis thaliana 80S cytosolic ribosome.
Article in The Biochemical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Systematic Review of Plant Ribosome Heterogeneity and Specialization.Frontiers in plant science · 2020Pooled it
- Ribosome Heterogeneity in Plants: The Causes of This Phenomenon and Its Implications on Gene Expression.Plants (Basel, Switzerland) · 2026Review
- TheProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Ribosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complex.Nature communications · 2025Article
- Review
- Remodelled ribosomal populations synthesize a specific proteome in proliferating plant tissue during cold.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Plant ribosomes as a score to fathom the melody of 2'-RNA biology · 2024Review
- Isolation of Cytosolic Ribosomes Associated with Plant Mitochondria and Chloroplasts.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Patterns of protein synthesis in the budding yeast cell cycle: variable or constant?Microbial cell (Graz, Austria) · 2024Article
- Proteomic profile of the germinating seeds reveals enhanced seedling growth in Arabidopsis rpp1a mutant.Plant molecular biology · 2023Article
- Cell-specific RNA profiling reveals host genes expressed in Arabidopsis cells haustoriated by downy mildew.Plant physiology · 2023Article
- FRIENDLY (FMT) is an RNA binding protein associated with cytosolic ribosomes at the mitochondrial surface.The Plant journal : for cell and molecular biology · 2022Article
- Engineering Ribosomes to Alleviate Abiotic Stress in Plants: A Perspective.Plants (Basel, Switzerland) · 2022Article
- A split green fluorescent protein system to enhance spatial and temporal sensitivity of translating ribosome affinity purification.The Plant journal : for cell and molecular biology · 2022Article
- Upon heat stress processing of ribosomal RNA precursors into mature rRNAs is compromised after cleavage at primary P site inRNA biology · 2022Article
- Diurnal dynamics of the Arabidopsis rosette proteome and phosphoproteome.Plant, cell & environment · 2021Article
- Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation.Scientific reports · 2021Article
- Increased Wheat Protein Content via Introgression of an HMW Glutenin Selectively Reshapes the Grain Proteome.Molecular & cellular proteomics : MCP · 2021Article
- Pollen Number and Ribosome Gene Expression Altered in a Genome-Editing Mutant ofFrontiers in plant science · 2021Article
- Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems.Frontiers in plant science · 2021Article
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5 authors at 1 institution in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Cytosolic 80S ribosomes contain proteins of the mature cytosolic ribosome (r-proteins) as well as proteins with roles in ribosome biogenesis, protein folding or modification. Here, we refined the core r-protein composition in Arabidopsis thaliana by determining the abundance of different proteins during enrichment of ribosomes from cell cultures using peptide mass spectrometry. The turnover rates of 26 40S subunit r-proteins and 29 60S subunit r-proteins were also determined, showing that half of the ribosome population is replaced every 3-4 days. Three enriched proteins showed significantly shorter half-lives; a protein annotated as a ribosomal protein uL10 (RPP0D, At1g25260) with a half-life of 0.5 days and RACK1b and c with half-lives of 1-1.4 days. The At1g25260 protein is a homologue of the human Mrt4 protein, a trans-acting factor in the assembly of the pre-60S particle, while RACK1 has known regulatory roles in cell function beyond its role in the 40S subunit. Our experiments also identified 58 proteins that are not from r-protein families but co-purify with ribosomes and co-express with r-proteins; 26 were enriched more than 10-fold during ribosome enrichment. Some of these enriched proteins have known roles in translation, while others are newly proposed ribosome-associated factors in plants. This analysis provides an improved understanding of A. thaliana ribosome protein content, shows that most r-proteins turnover in unison in vivo, identifies a novel set of potential plant translatome components, and how protein turnover can help identify r-proteins involved in ribosome biogenesis or regulation in plants.
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