Evidence map›Paper›PMID 32743578›Full record

ArticlebioRxiv : the preprint server for biology2020

Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.

Peng Zhao, Jeremy L Praissman, Oliver C Grant, Yongfei Cai, Tianshu Xiao, Katelyn E Rosenbalm, Kazuhiro Aoki, Benjamin P Kellman, Robert Bridger, Dan H Barouch and 6 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 48 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 1 country.

Peng ZhaoComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Jeremy L PraissmanComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Oliver C GrantComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Yongfei CaiDivision of Molecular Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA.
Tianshu XiaoDivision of Molecular Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA.
Katelyn E RosenbalmComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Kazuhiro AokiComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Benjamin P KellmanDepartments of Pediatrics and Bioengineering, University of California, San Diego, La Jolla, California, 92093, USA.
Robert BridgerComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Dan H BarouchCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, 02215, USA.
Melinda A BrindleyDepartment of Infectious Diseases, Department of Population Health, Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Nathan E LewisDepartments of Pediatrics and Bioengineering, University of California, San Diego, La Jolla, California, 92093, USA.
Michael TiemeyerComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Bing ChenDivision of Molecular Medicine, Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA.
Robert J WoodsComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
Lance WellsComplex Carbohydrate Research Center, Department of Biochemistry and Molecular Biology, and Department of Chemistry, University of Georgia, Athens, Georgia, 30602, USA.
University of Georgia · USBoston Children's Hospital · USBeth Israel Deaconess Medical Center · USFoundation Center · USUniversity of California San Diego · US

Funding

TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY W. · 2012 to 2019
$14.5M
TR&D1: Stem Cell and Induced Pluripotent Stem Cell Resources (Pages 116-134)P41GM103490 · NIGMS · UNIVERSITY OF GEORGIA · PI PIERCE, J. MICHAEL · 2012 to 2018
$13.7M
GlyGen Supplement: Develop automatic literature mining tool for extracting context specific glycan-protein data that will enhance the extent and quality of data in GlyGenU01GM125267 · NIGMS · UNIVERSITY OF GEORGIA · PI MAZUMDER, RAJA, TIEMEYER, MICHAEL · 2017 to 2021
$10.2M
Unraveling the mammalian secretory pathway through systems biology and algorithm developmentR35GM119850 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LEWIS, NATHAN ENOCH · 2016 to 2025
$4.3M
Origin of N-Glycan Site-Specific HeterogeneityR01GM130915 · NIGMS · UNIVERSITY OF GEORGIA · PI KANNAN, NATARAJAN, MOREMEN, KELLEY W. · 2019 to 2022
$3.4M
Structure of the full-length spike protein of SARS-CoV-2 in the context of membraneR01AI147884 · NIAID · BOSTON CHILDREN'S HOSPITAL · PI CHEN, BING · 2020 to 2024
$3.4M
Defining the role of phosphatidylserine in hemorrhagic fever virus replicationR01AI139238 · NIAID · UNIVERSITY OF GEORGIA · PI BRINDLEY, MELINDA ANN · 2019 to 2023
$2.0M
Tools to enable non-specialists to model glycoconjugates and glycan-protein interactionsU01CA207824 · NCI · UNIVERSITY OF GEORGIA · PI FOLEY, B. LACHELE, WOODS, ROBERT J · 2016 to 2018
$1.7M
NCI NIH HHS U01 CA207824NIAID NIH HHS R01 AI139238NIAID NIH HHS R01 AI147884NIGMS NIH HHS P41 GM103390NIGMS NIH HHS P41 GM103490NIGMS NIH HHS R01 GM130915NIGMS NIH HHS R35 GM119850NIGMS NIH HHS U01 GM125267
6 · The paper itself

Abstract

The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry. We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2. We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another. Our results highlight roles for glycans in sterically masking polypeptide epitopes and directly modulating Spike-ACE2 interactions. Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.

Indexed as

3D-modelingACE2coronavirusCOVID-19glycoproteinglycosylationmass spectrometrymolecular dynamicsSARS-CoV-2Spike protein

Identifiers

PMID32743578
PMCPMC7386495
OpenAlexW3037800945

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.