Evidence map›Paper›PMID 32742773›Full record

ArticlePeerJ2020

An updated genetic marker for detection of Lake Sinai Virus and metagenetic applications.

Deborah D Iwanowicz, Judy Y Wu-Smart, Tugce Olgun, Autumn H Smart, Clint R V Otto, Dawn Lopez, Jay D Evans, Robert Cornman

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Deborah D IwanowiczLeetown Science Center, U.S. Geological Survey, Kearneysville, WV, United States of America.
Judy Y Wu-SmartEntomology, University of Nebraska-Lincoln, Lincoln, NE, United States of America.
Tugce OlgunEntomology, University of Nebraska-Lincoln, Lincoln, NE, United States of America.
Autumn H SmartEntomology, University of Nebraska-Lincoln, Lincoln, NE, United States of America.
Clint R V OttoNorthern Prairie Wildlife Research Center, U.S. Geological Survey, Jamestown, ND, United States of America.
Dawn LopezBeltsville Agricultural Research Center, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD, United States of America.
Jay D EvansBeltsville Agricultural Research Center, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD, United States of America.
Robert CornmanFort Collins Science Center, United States Geological Survey, Fort Collins, CO, United States of America.
United States Geological Survey · USUniversity of Nebraska–Lincoln · USAgricultural Research Service · USBeltsville Agricultural Research Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLake Sinai Viruses (LSV) are common RNA viruses of honey bees (

methodsHere we report a new polymerase chain reaction (PCR) assay that is compatible with currently known lineages with minimal primer degeneracy, producing an expected 365 bp amplicon suitable for end-point PCR and metagenetic sequencing. Using the Illumina MiSeq platform, we performed pilot metagenetic assessments of three sample sets, each representing a distinct variable that might structure LSV diversity (geography, tissue, and species).

resultsThe first sample set in our pilot assessment compared cDNA pools from managed

Indexed as

Apis melliferaHalictus ligatusLake Sinai VirusMetageneticsPollinator

Identifiers

PMID32742773
PMCPMC7370930
OpenAlexW3042746727

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.