ArticleThe journal of venomous animals and toxins including tropical diseases2020
Suppression of cardiomyocyte functions by β-CTX isolated from the Thai king cobra (
Article in The journal of venomous animals and toxins including tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Sinus bradycardia and acute renal injury secondary to Viperidae snakebite: first case report.Frontiers in medicine · 2025Article
- Article
- S- and P-type cobra venom cardiotoxins differ in their action on isolated rat heart.The journal of venomous animals and toxins including tropical diseases · 2022Article
- Negative inotropic mechanisms of β-cardiotoxin in cardiomyocytes by depression of myofilament ATPase activity without activation of the classical β-adrenergic pathway.Scientific reports · 2021Article
- King Cobra and snakebite envenomation: on the natural history, human-snake relationship and medical importance ofThe journal of venomous animals and toxins including tropical diseases · 2021Review
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9 authors.
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Abstract
backgroundBeta-cardiotoxin (β-CTX), the three-finger toxin isolated from king cobra (
methodsβ-CTX was isolated from the crude venom of the Thai king cobra using reverse-phased and cation exchange HPLC.
resultsPurified β-CTX was recovered from crude venom (0.53% w/w). MTT assays revealed 50% cytotoxicity on A7r5 cells at 9.41 ± 1.14 µM (n = 3), but no cytotoxicity on C2C12 and H9c2 cells up to 114.09 µM. β-CTX suppressed the extend of rat cardiac cell shortening in a dose-dependent manner; the half-maximal inhibition concentration was 95.97 ± 50.10 nM (n = 3). In addition, the rates of cell shortening and re-lengthening were decreased in β-CTX treated myocytes concomitant with a prolongation of the intracellular calcium transient decay, indicating depression of cardiac contractility secondary to altered cardiac calcium homeostasis.
conclusionWe present an alternative purification method for β-CTX from king cobra venom. We reveal cytotoxicity towards smooth muscle and depression of cardiac contractility by this protein. These data are useful to aid future development of pharmacological agents derived from β-CTX.
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