ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2020
OMIP-068: High-Dimensional Characterization of Global and Antigen-Specific B Cells in Chronic Infection.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- HIV causes global B cell dysregulation and restricts HBV-specific B cell development in an incident HBV cohort.The Journal of clinical investigation · 2026Observational
- Heavy-chain immunoglobulin locus editing in rhesus macaque B cells to confer antibody production.Molecular therapy. Methods & clinical development · 2025Article
- Circulating B cells display differential immune regulatory molecule expression in granulomatosis with polyangiitis.Clinical and experimental immunology · 2025Article
- Circulating HBsAg-specific B cells are partially rescued in chronically HBV-infected patients with functional cure.Emerging microbes & infections · 2024Article
- Comprehensive Immunophenotyping by Polychromatic Cytometry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Side-by-Side Comparison of Compensation Beads Used in Polychromatic Flow Cytometry.ImmunoHorizons · 2023Article
- Deep Immunophenotyping of Human Whole Blood by Standardized Multi-parametric Flow Cytometry Analyses.Phenomics (Cham, Switzerland) · 2023Article
- Adenoid lymphocyte heterogeneity in pediatric adenoid hypertrophy and obstructive sleep apnea.Frontiers in immunology · 2023Article
- Increased death and exhaustion of CD69PeerJ · 2023Article
- Comprehensive phenotyping of human peripheral blood B lymphocytes in pathological conditions.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).European journal of immunology · 2021Review
- Characterising the Phenotypic Diversity of Antigen-Specific Memory B Cells Before and After Vaccination.Frontiers in immunology · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
This 24-color flow cytometry panel focuses on characterizing antigen-specific B cells and precise delineation of B-cell subsets in chronic infections and is applicable to other chronic diseases such as autoimmunity. The panel was optimized for human cryopreserved peripheral blood mononuclear cells (PBMCs). Markers were chosen to extensively distinguish B-cell lineages (CD19, CD20, CD10, CD38, CD24, IgM, IgD, CD27, CD21, CD43, CD5). Inclusion of antigen-specific probes was of high priority in order to assess hepatitis B virus (HBV) antigen-specific B cells for our purposes. These probes can be readily exchanged for other pathogen-specific probes or additional markers for the panel to be tailored to desired research questions beyond HBV. In addition, we included a comprehensive and unique set of functional markers such as chemokine receptors (CXCR3, CXCR5), co-stimulatory molecule (CD86), Fc receptor (CD32), regulatory molecules (BTLA, CD39), and inhibitory markers associated with chronic infections (PD-1, FcRL5, CD11c, CD22) to enable in-depth analysis of global and antigen-specific B cells during chronic infection. © 2020 International Society for Advancement of Cytometry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.