ArticlePlant biotechnology journal2021
A novel strategy for creating a new system of third-generation hybrid rice technology using a cytoplasmic sterility gene and a genic male-sterile gene.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 72 citations in OpenAlex.
- Fine mapping and candidate gene analysis of tassel glume closure1 (tgc1) gene in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Innovations in hybrid rice seed production: Integrating agronomy, mechanization, and biotechnology.Plant communications · 2026Review
- An all-in-one visual selection system for male-sterile line production in maize and rice.Plant communications · 2026Article
- Unraveling Advances in Rice Male Sterility Systems: From Genetic Basis to Hybrid Breeding Innovation.Plants (Basel, Switzerland) · 2026Review
- Advancements in genomic crop techniques and considerations for regulation and food safety.Transgenic research · 2025Review
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- Identification and Characterization of a Male Sterile Rapeseed (Plants (Basel, Switzerland) · 2025Article
- Targeted mutation ofHorticulture research · 2025Article
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- Review
- Genetic characterization and fine mapping of a recessive genic male-sterile gene in flowering Chinese cabbage (3 Biotech · 2024Article
- Identification of two plastid transit peptides for construction of pollen-inactivation system in rice.Molecular breeding : new strategies in plant improvement · 2024Article
- Research on Physiological Characteristics and Differential Gene Expression of Rice Hybrids and Their Parents under Salt Stress at Seedling Stage.Plants (Basel, Switzerland) · 2024Article
- Hybrid Rice Production: A Worldwide Review of Floral Traits and Breeding Technology, with Special Emphasis on China.Plants (Basel, Switzerland) · 2024Review
- Review
- Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene.Rice (New York, N.Y.) · 2024Article
- Establishment and Advances of Third-Generation Hybrid Rice Technology: A Review.Rice (New York, N.Y.) · 2023Review
- Glutaredoxin in Rice Growth, Development, and Stress Resistance: Mechanisms and Research Advances.International journal of molecular sciences · 2023Review
- CRISPR/Cas Technology Revolutionizes Crop Breeding.Plants (Basel, Switzerland) · 2023Review
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
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Authors and funding
16 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterosis utilization is the most effective way to improve rice yields. The cytoplasmic male-sterility (CMS) and photoperiod/thermosensitive genic male-sterility (PTGMS) systems have been widely used in rice production. However, the rate of resource utilization for the CMS system hybrid rice is low, and the hybrid seed production for the PTGMS system is affected by the environment. The technical limitations of these two breeding methods restrict the rapid development of hybrid rice. The advantages of the genic male-sterility (GMS) rice, such as stable sterility and free combination, can fill the gaps of the first two generations of hybrid rice technology. At present, the third-generation hybrid rice breeding technology is being used to realize the application of GMS materials in hybrid rice. This study aimed to use an artificial CMS gene as a pollen killer to create a smart sterile line for hybrid rice production. The clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) technology was used to successfully obtain a CYP703A3-deficient male-sterile mutant containing no genetically modified component in the genetic background of indica 9311. Through young ear callus transformation, this mutant was transformed with three sets of element-linked expression vectors, including pollen fertility restoration gene CYP703A3, pollen-lethality gene orfH79 and selection marker gene DsRed2. The maintainer 9311-3B with stable inheritance was obtained, which could realize the batch breeding of GMS materials. Further, the sterile line 9311-3A and restorer lines were used for hybridization, and a batch of superior combinations of hybrid rice was obtained.
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