Evidence map›Paper›PMID 32740927›Full record

ArticleBiopolymers2021

Side-chain thioamides as fluorescence quenching probes.

D Miklos Robkis, Eileen M Hoang, Pengse Po, Carol J Deutsch, E James Petersson

Abstract read
In one paragraph

Article in Biopolymers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

D Miklos RobkisDepartment of Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Eileen M HoangDepartment of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Pengse PoDepartment of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Carol J DeutschDepartment of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
E James PeterssonDepartment of Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.ORCID https://orcid.org/0000-0003-3854-9210

Funding

FUNCTION AND ASSEMBLY OF POTASSIUM CHANNELSR01GM052302 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI DEUTSCH, CAROL J · 1995 to 2024
$13.4M
Time-Of-Flight Instrument with Variable Ionization SourcesS10RR023444 · NCRR · UNIVERSITY OF PENNSYLVANIA · PI DMOCHOWSKI, IVAN JULIAN · 2007 to 2007
$308k
National Science Foundation NSF CHE-1150351National Science Foundation NSF CHE-1708759National Science Foundation NSF CHE-1827457National Science Foundation NSF MRI-0820996NCRR NIH HHS S10 RR023444NIGMS NIH HHS R01 GM052302NIH HHS NIH R01-GM052302NIH HHS NIH RR-023444
6 · The paper itself

Abstract

Thioamides, single atom oxygen-to-sulfur substitutions of canonical amide bonds, can be valuable probes for protein folding and protease studies. Here, we investigate the fluorescence quenching properties of thioamides incorporated into the side-chains of amino acids. We synthesize and incorporate Fmoc-protected, solid-phase peptide synthesis building blocks for introducing N

Indexed as

Amino AcidsFluorescence Resonance Energy TransferFluorescent DyesPeptidesSolid-Phase Synthesis TechniquesThioamidesAmino AcidsFluorescent DyesPeptidesThioamidesfluorescenceFRETquenchingthioamideunnatural amino acid

Identifiers

PMID32740927
PMCPMC7744324

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.