Evidence map›Paper›PMID 32740713›Full record

ArticleCurrent microbiology2020

Impact of Temperature, Nutrients and Heavy Metals on Bacterial Diversity and Ecosystem Functioning Studied by Freshwater Microcosms and High-Throughput DNA Sequencing.

Tianma Yuan, Alan J McCarthy, Yixin Zhang, Raju Sekar

Abstract read
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In one paragraph

Article in Current microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. A latest review on the application of microcosm model in environmental research.Environmental science and pollution research international · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tianma YuanDepartment of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, 215123, Jiangsu, China.
Alan J McCarthyMicrobiology Research Group, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
Yixin ZhangResearch Center of Environmental Protection and Ecological Restoration Technology, Department of Landscape Architecture, Gold Mantis School of Architecture, Soochow University, Suzhou, Jiangsu, China.
Raju SekarDepartment of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, 215123, Jiangsu, China. Sekar.Raju@xjtlu.edu.cn.ORCID http://orcid.org/0000-0002-1182-9004

Funding

Natural Science Foundation of Jiangsu Province BK20171238Natural Science Foundation of the Jiangsu Higher Education Institutions of China 13KJB180022Xi'an Jiaotong-Liverpool University KSF-E-20Xi'an Jiaotong-Liverpool University PGRS-13-03-09
6 · The paper itself

Abstract

Microbial communities are fundamental components in freshwater, and community shifts in ecosystem structure are indicative of changing environmental conditions. This study aimed at investigating the influence of key environmental parameters on bacterial diversity and ecosystem functioning (i.e. organic matter breakdown) in laboratory freshwater microcosms. The effects of varying temperatures (5, 20 and 35 °C), nutrients (representing low, medium and high urbanization) and heavy metals Copper (Cu) and Zinc (Zn) on bacterial diversity and organic matter (OM) breakdown were studied by using leaf bags and capsules filled with polycaprolactonediol-2000 (PCP-2000), respectively. The leaf-associated bacterial diversity was determined by next-generation sequencing of SSU rRNA gene amplicons. The results showed that bacterial diversity increased at high temperature (35 °C) with more operational taxonomic units (OTUs) as compared to medium (20 °C) or low (5 °C) temperatures, whereas nutrient variation had fewer effects on the bacterial community structure. In contrast, the presence of heavy metals, especially high concentrations (100 μM) of Cu, reduced the number of OTUs in the leaf-associated bacterial community. The higher temperatures and nutrient levels accelerated PCP-2000 breakdown rate, but this was impeded by a high concentration (100 μM) of Cu in the short term, though no effect of Zn on breakdown rate was observed. The overall results indicate that temperature and variated heavy metals are among the key factors that affect bacterial diversity and ecosystem functioning in freshwater systems.

Indexed as

EcosystemMetals, HeavyFresh WaterHigh-Throughput Nucleotide SequencingNutrientsTemperatureMetals, HeavyNutrients

Identifiers

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.