Evidence map›Paper›PMID 32737835›Full record

ArticleIn vitro cellular & developmental biology. Animal2020

Screening of single-cell clonal lines from Papilio demoleus Linnaeus cell lines for exogenous protein expression and adaptation in serum-free culture.

Wei-Feng Ding, Zhi-Gang Liu, Na Sun, Xin Zhang, Xian Li, Ying Feng

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Wei-Feng DingResearch Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650233, Yunnan, China.
Zhi-Gang LiuResearch Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650233, Yunnan, China.
Na SunResearch Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650233, Yunnan, China.
Xin ZhangResearch Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650233, Yunnan, China.
Xian LiResearch Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650233, Yunnan, China.
Ying FengResearch Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming, 650233, Yunnan, China. rirify@139.com.ORCID http://orcid.org/0000-0002-2471-8071
Research Institute of Resource Insects · CN

Funding

Fundamental Research Funds for the Central Non-profit Research Institution of CAF CAFYBB2016ZD005
6 · The paper itself

Abstract

In this paper, four established cell lines derived from newly hatched larvae of Papilio demoleus Linnaeus and 57 single-cell clones derived from the 3 lines were used as test materials. Recombinant β-galactosidase baculovirus AcMNPV-Gal was used to infect the P. demoleus L. cell lines and the single-cell clones, and recombinant protein expression in each cell line was detected and compared. Three clonal cell lines, RIRI-PaDe-1-C1, RIRI-PaDe-2-C6 and RIRI-PaDe-3-C52, which showed significantly higher β-galactosidase expression levels than those of the parental cell lines, were selected. Five types of commercial serum-free media for insect cells, Express Five SFM, Ex-Cell 405, Sf-900III SFM, Sf-900II SFM and HyClone Serum-Free Media, were used to adapt RIRI-PaDe-2-C6 cells and RIRI-PaDe-3-C52 cells to serum-free culture conditions, and the growth characteristics of the cells and the exogenous protein expression characteristics before and after adaptation were compared. The results showed that RIRI-PaDe-2-C6 cells could stably proliferate in Ex-Cell 405, RIRI-PaDe-3-C52 cells could stably proliferate in Express Five SFM and Ex-Cell 405, and the rate of proliferation of and the level of expression of β-galactosidase in RIRI-PaDe-3-C52 cells were significantly increased in Express Five SFM.

Indexed as

Adaptation, PhysiologicalGene ExpressionSingle-Cell AnalysisAnimalsBaculoviridaebeta-GalactosidaseButterfliesCell LineCell ProliferationCell ShapeClone CellsCulture Media, Serum-FreeKineticsRecombinant Proteinsbeta-GalactosidaseCulture Media, Serum-FreeRecombinant ProteinsInsect cell linePapilio demoleusRecombinant protein expressionSingle-cell clone

Identifiers

PMID32737835
OpenAlexW3046883142

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.