ReviewNature protocols2020
Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy.
Review in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
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- Modular Parallel Plate Flow Chamber with Tunable Substrate Mechanics and Defined Shear Stress.bioRxiv : the preprint server for biology · 2025Article
- Ethyl Cinnamate-Based Tissue Clearing Technique on Vocal Fold Mucosa with Vasculature Model.Journal of voice : official journal of the Voice Foundation · 2025Article
- Near-isotropic super-resolution microscopy with axial interference speckle illumination.Nature communications · 2025Article
- Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet Fluorescence Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Cleared tissue dual-view oblique plane microscopy.Biomedical optics express · 2025Article
- Standardized measurements for monitoring and comparing multiphoton microscope systems.Nature protocols · 2025Review
- Strategy for Generating Giant Unilamellar Vesicles with Tunable Size Using the Modified cDICE Method.ACS synthetic biology · 2025Article
- Cellular and biophysical barriers to lipid nanoparticle mediated delivery of RNA to the cytosol.Nature communications · 2025Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Colony context and size-dependent compensation mechanisms give rise to variations in nuclear growth trajectories.Cell systems · 2025Article
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- Symplastic guard cell connections buffer pressure fluctuations to promote stomatal function in grasses.The New phytologist · 2025Article
- Micro Immune Response On-chip (MIRO) models the tumour-stroma interface for immunotherapy testing.Nature communications · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Spherical aberration (SA) occurs when light rays entering at different points of a spherical lens are not focused to the same point of the optical axis. SA that occurs inside the lens elements of a fluorescence microscope is well understood and corrected for. However, SA is also induced when light passes through an interface of refractive index (RI)-mismatched substances (i.e., a discrepancy between the RI of the immersion medium and the RI of the sample). SA due to RI mismatches has many deleterious effects on imaging. Perhaps most important for 3D imaging is that the distance the image plane moves in a sample is not equivalent to the distance traveled by an objective (or stage) during z-stack acquisition. This non-uniform translation along the z axis gives rise to artifactually elongated images (if the objective is immersed in a medium with a higher RI than that of the sample) or compressed images (if the objective is immersed in a medium with a lower RI than that of the sample) and alters the optimal axial sampling rate. In this tutorial, we describe why this distortion occurs, how it impacts quantitative measurements and axial resolution, and what can be done to avoid SA and thereby prevent distorted images. In addition, this tutorial aims to better inform researchers of how to correct RI mismatch-induced axial distortions and provides a practical ImageJ/Fiji-based tool to reduce the prevalence of volumetric measurement errors and lost axial resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.