ArticleNPJ biofilms and microbiomes2020
Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens.
Article in NPJ biofilms and microbiomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 30 citations in OpenAlex.
- Biofilm Formation and Spore-Mediated Persistence of Clostridium perfringens in Meat and Poultry Processing Environments and Their Implications for Control Strategies.Journal of food science · 2026Review
- A genetic toolkit for the human gut bacterium Mediterraneibacter gnavus identifies capsular polysaccharides as a competitive colonization factor.Nature communications · 2026Article
- Plastisphere-isolatedFrontiers in microbiology · 2026Article
- Thermal modulation of transcriptional states, biofilm architecture and survival strategies inFrontiers in microbiology · 2026Article
- Host-microbe interactions characterized by gene expression of cervical adhesion molecules, cytokines, and growth factors define the recurrence of bacterial vaginosis.Research square · 2025Article
- Microscopic Analysis of Temperature Effects on Surface Colonization and Biofilm Morphology ofFoods (Basel, Switzerland) · 2025Article
- The Effect of Temperature over the Growth and Biofilm Formation of the ThermotolerantJournal of fungi (Basel, Switzerland) · 2025Article
- AlleviatingAnimals : an open access journal from MDPI · 2025Article
- The phototrophic purple non-sulfur bacteria Rhodomicrobium spp. are novel chassis for bioplastic production.Microbial biotechnology · 2024Article
- Genome-encoded ABCF factors implicated in intrinsic antibiotic resistance in Gram-positive bacteria: VmlR2, Ard1 and CplR.Nucleic acids research · 2023Article
- Electroactive biofilms: how microbial electron transfer enables bioelectrochemical applications.Journal of industrial microbiology & biotechnology · 2022Review
- Development of Desiccation-Tolerant Probiotic Biofilms Inhibitory for Growth of Foodborne Pathogens on Stainless Steel Surfaces.Foods (Basel, Switzerland) · 2022Article
- Non-deacetylated poly-Frontiers in microbiology · 2022Article
- Assessment of the Impact of Temperature on Biofilm Composition with a Laboratory Heat Exchanger Module.Microorganisms · 2021Article
- Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells in biofilms dynamically adapt to surrounding environmental conditions, which alters biofilm architecture. The obligate anaerobic pathogen Clostridium perfringens shows different biofilm structures in different temperatures. Here we find that the temperature-regulated production of extracellular polymeric substance (EPS) is necessary for morphological changes in biofilms. We identify BsaA proteins as an EPS matrix necessary for pellicle biofilm formation at lower temperature and find that extracellularly secreted BsaA protein forms filamentous polymers. We show that sipW-bsaA operon expression is bimodal, and the EPS-producing population size is increased at a lower temperature. This heterogeneous expression of the EPS gene requires a two-component system. We find that EPS-producing cells cover EPS-nonproducing cells attaching to the bottom surface. In the deletion mutant of pilA2, encoding a type IV pilin, the EPS gene expression is ON in the whole population. This heterogeneity is further regulated by the cleavage of the pilA2 mRNA by RNase Y, causing temperature-responsive EPS expression in biofilms. As temperature is an environmental cue, C. perfringens may modulate EPS expression to induce morphological changes in biofilm structure as a strategy for adapting to interhost and external environments.
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