ArticleProceedings of the National Academy of Sciences of the United States of America2020
Protein nanoribbons template enamel mineralization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 74 citations in OpenAlex.
- Serine-16 Phosphorylation, C-Terminal Truncation, and Ion-Specific Interactions Coordinate Amelogenin Nanoribbon Formation.Calcified tissue international · 2026Article
- Amelogenin proteolysis orchestrates functional amyloid pathways in enamel development.Matrix biology : journal of the International Society for Matrix Biology · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Review
- Biomimetic supramolecular protein matrix restores structure and properties of human dental enamel.Nature communications · 2025Article
- Amyloid-induced mineralization: From biological systems to biomimetic materials.Bioactive materials · 2025Review
- Design Principles Of Inorganic-Protein Hybrid Materials for Biomedicine.Exploration (Beijing, China) · 2025Review
- Identification and elemental mapping of enamel minerals with electron energy-loss spectroscopy.RSC advances · 2025Article
- Ameloblastin binding to biomimetic models of cell membranes - A continuum of intrinsic disorder.Archives of oral biology · 2025Article
- Amyloid and collagen templates in aortic valve calcification.Trends in molecular medicine · 2024Article
- Hybrid Hydroxyapatite-Metal Complex Materials Derived from Amino Acids and Nucleobases.Molecules (Basel, Switzerland) · 2024Review
- Recombinant human enamelin produced in Escherichia coli promotes mineralization in vitro.BMC biotechnology · 2024Article
- Biomineral-Based Composite Materials in Regenerative Medicine.International journal of molecular sciences · 2024Review
- Occlusive membranes for guided regeneration of inflamed tissue defects.Nature communications · 2023Article
- A multiple Kirkendall strategy for converting nanosized zero-valent iron to highly active Fenton-like catalyst for organics degradation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- An epigallocatechin gallate-amorphous calcium phosphate nanocomposite for caries prevention and demineralized enamel restoration.Materials today. Bio · 2023Article
- The contribution of amyloid deposition in the aortic valve to calcification and aortic stenosis.Nature reviews. Cardiology · 2023Review
- Resolving protein-mineral interfacial interactions duringMaterials today. Advances · 2023Article
- Biomimetic Mineral Synthesis by Nanopatterned Supramolecular-Block Copolymer Templates.Nano letters · 2023Article
- Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development.Stem cell reports · 2023Article
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8 authors at 2 institutions in 2 countries.
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Abstract
As the hardest tissue formed by vertebrates, enamel represents nature's engineering masterpiece with complex organizations of fibrous apatite crystals at the nanometer scale. Supramolecular assemblies of enamel matrix proteins (EMPs) play a key role as the structural scaffolds for regulating mineral morphology during enamel development. However, to achieve maximum tissue hardness, most organic content in enamel is digested and removed at the maturation stage, and thus knowledge of a structural protein template that could guide enamel mineralization is limited at this date. Herein, by examining a gene-modified mouse that lacked enzymatic degradation of EMPs, we demonstrate the presence of protein nanoribbons as the structural scaffolds in developing enamel matrix. Using in vitro mineralization assays we showed that both recombinant and enamel-tissue-based amelogenin nanoribbons are capable of guiding fibrous apatite nanocrystal formation. In accordance with our understanding of the natural process of enamel formation, templated crystal growth was achieved by interaction of amelogenin scaffolds with acidic macromolecules that facilitate the formation of an amorphous calcium phosphate precursor which gradually transforms into oriented apatite fibers along the protein nanoribbons. Furthermore, this study elucidated that matrix metalloproteinase-20 is a critical regulator of the enamel mineralization as only a recombinant analog of a MMP20-cleavage product of amelogenin was capable of guiding apatite mineralization. This study highlights that supramolecular assembly of the scaffold protein, its enzymatic processing, and its ability to interact with acidic carrier proteins are critical steps for proper enamel development.
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