ArticleParasites & vectors2020
First multilocus sequence typing (MLST) of Giardia duodenalis isolates from humans in Romania.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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17 citing papers in PubMed, 26 citations in OpenAlex.
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- Prevalence and Genotyping of Water- and Food-Borne Parasitic Protozoans (Microorganisms · 2024Article
- Molecular Epidemiology and Assemblage Typing of Giardia duodenalis in School-Age Children Situated along the Southern Shoreline of Lake Malawi, Malawi.The American journal of tropical medicine and hygiene · 2023Article
- Role of rodents in the zoonotic transmission of giardiasis.One health (Amsterdam, Netherlands) · 2023Review
- Assessment of genetic markers for multilocus sequence typing (MLST) of Fasciola isolates from Iran.Veterinary medicine and science · 2023Article
- Influence of Selected Factors on the Survival Assessment and Detection ofPathogens (Basel, Switzerland) · 2023Article
- Global molecular prevalence ofHeliyon · 2023Review
- Giardia duodenalis in patients with diarrhea and various animals in northeastern China: prevalence and multilocus genetic characterization.Parasites & vectors · 2022Article
- Multilocus sequence typing ofFrontiers in medicine · 2022Article
- Zoonotic giardiasis: an update.Parasitology research · 2021Review
- Correction to: First multilocus sequence typing (MLST) of Giardia duodenalis isolates from humans in Romania.Parasites & vectors · 2021Article
- Genetic Diversity and Prevalence ofFrontiers in cellular and infection microbiology · 2021Article
- The controversies surroundingCurrent research in parasitology & vector-borne diseases · 2021Review
- Risk Factor ofIranian journal of parasitologyArticle
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiardia duodenalis is one of the most prevalent and highly diverse human parasites, encompassing a complex of eight genetically distinct assemblages, each further divided into sub-assemblages. While in recent years, G. duodenalis genotype distribution patterns in humans have been intensely studied, there is still very little information available on the diversity of Giardia genotypes and sub-assemblages infecting people in Romania. In the present study, we investigated the genetic diversity of Giardia duodenalis in asymptomatic patients from Romania.
methodsOver an 11-month period, human feces from 7805 healthy adults were screened by microscopic analysis for G. duodenalis cysts during their obligatory periodic check-ups. DNA extraction was performed from microscopic-positive fecal samples, followed by multilocus sequence typing of four genetic loci of the ITS region, gdh, tpi and bg genes, followed by DNA sequencing and phylogenetic analysis. Statistical analysis was performed using EpiInfo 2000 software.
resultsThe prevalence of giardiasis in the present study was 0.42% (33/7805). Twenty-three samples (76.67%) were successfully genotyped at each locus. The bg and tpi genes had the highest typing success rate (100%). The identified assemblages were assemblage A in 27 cases (subtypes A2 and A3), and B in 3 cases.
conclusionsTo our knowledge, the present study is the first report of multilocus sequence typing of G. duodenalis isolated from humans in Romania. The present results may shed light on G. duodenalis infection in humans at a regional and national level, thus increasing awareness against this parasitic infection.
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