Evidence map›Paper›PMID 32736581›Full record

ArticleParasites & vectors2020

Fluorescent bead-based serological detection of Toxoplasma gondii infection in chickens.

Benedikt T Fabian, Fatima Hedar, Martin Koethe, Berit Bangoura, Pavlo Maksimov, Franz J Conraths, Isabelle Villena, Dominique Aubert, Frank Seeber, Gereon Schares

Open access · goldAbstract read
In one paragraph

Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.7field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Molecular prevalence and genetic diversity ofOpen veterinary journal · 2023
    Article
  2. Review
  3. Foods (Basel, Switzerland) · 2022
    Review
  4. Article
  5. Prevalence ofJournal of veterinary research · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 3 countries.

Benedikt T FabianFG16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute, Berlin, Germany.
Fatima HedarFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, National Reference Centre for Toxoplasmosis, Greifswald-Insel Riems, Germany.
Martin KoetheFaculty of Veterinary Medicine, Institute of Food Hygiene, Leipzig University, Leipzig, Germany.
Berit BangouraFaculty of Veterinary Medicine, Institute of Parasitology, Leipzig University, Leipzig, Germany.
Pavlo MaksimovFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, National Reference Centre for Toxoplasmosis, Greifswald-Insel Riems, Germany.
Franz J ConrathsFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, National Reference Centre for Toxoplasmosis, Greifswald-Insel Riems, Germany.
Isabelle VillenaEA 7510, UFR Medecine, University of Reims Champagne Ardenne, Reims, France.
Dominique AubertEA 7510, UFR Medecine, University of Reims Champagne Ardenne, Reims, France.
Frank SeeberFG16: Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute, Berlin, Germany.
Gereon ScharesFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, National Reference Centre for Toxoplasmosis, Greifswald-Insel Riems, Germany. gereon.schares@fli.de.ORCID http://orcid.org/0000-0002-3217-289X
Friedrich-Loeffler-Institut · DECentre Hospitalier Universitaire de Reims · FRRobert Koch Institute · DELeipzig University · DEUniversity of Wyoming · US

Funding

Bundesministerium für Bildung und Forschung 01KI0765Bundesministerium für Bildung und Forschung 01KI1002FBundesministerium für Forschung und Technologie 01KI1002CDeutsche Forschungsgemeinschaft Project 251133687/ GRK 2046Horizon 2020 773830
6 · The paper itself

Abstract

backgroundFree-ranging chickens are often infected with Toxoplasma gondii and seroconvert upon infection. This indicates environmental contamination with T. gondii.

methodsHere, we established a bead-based multiplex assay (BBMA) using the Luminex technology for the detection of T. gondii infections in chickens. Recombinant biotinylated T. gondii surface antigen 1 (TgSAG1

resultsIn experimentally infected chickens, the vast majority (98.5%, n = 65/66) of birds tested seropositive in the BBMA. This included all chickens positive by magnetic-capture PCR (100%, n = 45/45). Most, but not all inoculated and TgSAG1

conclusionsThe TgSAG1

Indexed as

AnimalsAntigens, ProtozoanChickensEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectPoultry DiseasesSerologic TestsToxoplasmaToxoplasmosis, AnimalAntigens, ProtozoanELISAIFATLuminex assayMagnetic-Capture PCRMATMultiplexingReal-time PCRSAG1SerumToxoplasma gondii

Identifiers

PMID32736581
PMCPMC7393333
OpenAlexW3046899812

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.