Evidence map›Paper›PMID 32735908›Full record

ArticleExperimental hematology2020

Single-cell lineage tracing approaches in hematology research: technical considerations.

Joana Carrelha, Dawn S Lin, Alejo E Rodriguez-Fraticelli, Tiago C Luis, Adam C Wilkinson, Nina Cabezas-Wallscheid, Cedric S Tremblay, Simon Haas

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental hematology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Joana CarrelhaHaematopoietic Stem Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK; MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Dawn S LinImmunology, The Walter & Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Faculty of Medicine, Dentistry & Health Sciences, University of Melbourne, Parkville, Victoria, Australia.
Alejo E Rodriguez-FraticelliStem Cell Program, Boston Children's Hospital, Boston, Massachusetts, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA.
Tiago C LuisDepartment of Life Sciences, Imperial College London, London, UK.
Adam C WilkinsonInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
Nina Cabezas-WallscheidMax Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany.
Cedric S TremblayAustralian Centre for Blood Diseases, Central Clinical School, Monash University, Melbourne, Australia.
Simon HaasHeidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany; Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH Alliance, Heidelberg, Germany. Electronic address: s.haas@dkfz-heidelberg.de.

Funding

mechanisms of in situ functional stem cell heterogeneity in native and transplantation hematopoiesisK99HL146983 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI RODRIGUEZ FRATICELLI, ALEJO EZEQUIEL · 2019 to 2020
$336k
A synthetic hematopoietic stem cell niche to investigate stemness and hematopathologiesK99HL150218 · NHLBI · STANFORD UNIVERSITY · PI WILKINSON, ADAM C · 2020 to 2020
$167k
Medical Research Council MC_UU_12009/5NHLBI NIH HHS K99 HL146983NHLBI NIH HHS K99 HL150218Wellcome Trust
6 · The paper itself

Abstract

The coordinated differentiation of hematopoietic stem and progenitor cells (HSPCs) into the various mature blood cell types is responsible for sustaining blood and immune system homeostasis. The cell fate decisions underlying this important biological process are made at the level of single cells. Methods to trace the fate of single cells are therefore essential for understanding hematopoietic system activity in health and disease and have had a major impact on how we understand and represent hematopoiesis. Here, we discuss the basic methodologies and technical considerations for three important clonal assays: single-cell transplantation, lentiviral barcoding, and Sleeping Beauty barcoding. This perspective is a synthesis of presentations and discussions from the 2019 International Society for Experimental Hematology (ISEH) Annual Meeting New Investigator Technology Session and the 2019 ISEH Winter Webinar.

Indexed as

AnimalsCell DifferentiationCell LineageCell TrackingCell TransplantationCongresses as TopicDNA Barcoding, TaxonomicGenetic VectorsHematologyHematopoiesisHematopoietic Stem CellsHomeostasisHumansLentivirusMiceSingle-Cell Analysissleeping beauty transposase, humanTransposases

Identifiers

PMID32735908
PMCPMC7894992

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.