ArticleCurrent biology : CB2020
Highly Efficient Knockout of a Squid Pigmentation Gene.
Article in Current biology : CB, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Cephalopod Behavior: From Neural Plasticity to Consciousness.Frontiers in systems neuroscience · 2021Pooled it
- Efficient Microinjection and CRISPR/Cas9 Mediated Genome Editing in Urechis unicinctus.Marine biotechnology (New York, N.Y.) · 2026Article
- Article
- Maintaining microbiota across diverse symbiotic organs inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- A non-invasive method to genotype cephalopod sex by quantitative PCR.bioRxiv : the preprint server for biology · 2025Article
- Evo-Inspired Engineering of Radical Phenotypes and Emergent Traits.Integrative and comparative biology · 2025Review
- A genetically tractable non-vertebrate system to study complete camera-type eye regeneration.Nature communications · 2025Article
- Review
- Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata.EvoDevo · 2025Article
- The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Heredity · 2025Article
- Pigment granule architecture varies across yellow, red, and brown chromatophores in squid Doryteuthis pealeii.Scientific reports · 2024Article
- Comparative Analysis of Promoter Activity in Crassostrea gigas Embryos: Implications for Bivalve Gene Editing.Marine biotechnology (New York, N.Y.) · 2024Article
- Embryonic development of a centralised brain in coleoid cephalopods.Neural development · 2024Review
- A new dawn for the study of cell type evolution.Development (Cambridge, England) · 2024Review
- Efficient CRISPR genome editing and integrative genomic analyses reveal the mosaicism of Cas-induced mutations and pleiotropic effects ofbioRxiv : the preprint server for biology · 2024Article
- Gene modelling and annotation for the Hawaiian bobtail squid, Euprymna scolopes.Scientific data · 2024Article
- Cephalopod-omics: Emerging Fields and Technologies in Cephalopod Biology.Integrative and comparative biology · 2023Article
- Molluscan Genomes Reveal Extensive Differences in Photopigment Evolution Across the Phylum.Molecular biology and evolution · 2023Article
- Emerging questions on the mechanisms and dynamics of 3D genome evolution in spiralians.Briefings in functional genomics · 2023Review
- The neural basis of visual processing and behavior in cephalopods.Current biology : CB · 2023Review
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6 authors at 2 institutions in 1 country.
Funding
Abstract
Seminal studies using squid as a model led to breakthroughs in neurobiology. The squid giant axon and synapse, for example, laid the foundation for our current understanding of the action potential [1], ionic gradients across cells [2], voltage-dependent ion channels [3], molecular motors [4-7], and synaptic transmission [8-11]. Despite their anatomical advantages, the use of squid as a model receded over the past several decades as investigators turned to genetically tractable systems. Recently, however, two key advances have made it possible to develop techniques for the genetic manipulation of squid. The first is the CRISPR-Cas9 system for targeted gene disruption, a largely species-agnostic method [12, 13]. The second is the sequencing of genomes for several cephalopod species [14-16]. If made genetically tractable, squid and other cephalopods offer a wealth of biological novelties that could spur discovery. Within invertebrates, not only do they possess by far the largest brains, they also express the most sophisticated behaviors [17]. In this paper, we demonstrate efficient gene knockout in the squid Doryteuthis pealeii using CRISPR-Cas9. Ommochromes, the pigments found in squid retinas and chromatophores, are derivatives of tryptophan, and the first committed step in their synthesis is normally catalyzed by Tryptophan 2,3 Dioxygenase (TDO [18-20]). Knocking out TDO in squid embryos efficiently eliminated pigmentation. By precisely timing CRISPR-Cas9 delivery during early development, the degree of pigmentation could be finely controlled. Genotyping revealed knockout efficiencies routinely greater than 90%. This study represents a critical advancement toward making squid genetically tractable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.