ArticleCurrent biology : CB2020
Mechanochemical Crosstalk Produces Cell-Intrinsic Patterning of the Cortex to Orient the Mitotic Spindle.
Article in Current biology : CB, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Competition and synergy of Arp2/3 and formins in nucleating actin waves.Cell reports · 2024Article
- Mechanical stress combines with planar polarised patterning during metaphase to orient embryonic epithelial cell divisions.Development (Cambridge, England) · 2024Article
- On the origin of the nucleus: a hypothesis.Microbiology and molecular biology reviews : MMBR · 2023Review
- Competition and Synergy of Arp2/3 and Formins in Nucleating Actin Waves.bioRxiv : the preprint server for biology · 2023Article
- Oncogenic Ras deregulates cell-substrate interactions during mitotic rounding and respreading to alter cell division orientation.Current biology : CB · 2023Article
- A prognostic model based on the Augmin family genes for LGG patients.Scientific reports · 2023Article
- Deep learning techniques and mathematical modeling allow 3D analysis of mitotic spindle dynamics.The Journal of cell biology · 2023Article
- Mitotic outcomes and errors in fibrous environments.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Drosophila mitotic spindle orientation machinery requires activation, not just localization.EMBO reports · 2023Article
- Collective dynamics of actin and microtubule and its crosstalk mediated by FHDC1.Frontiers in cell and developmental biology · 2023Article
- Spindle reorientation in response to mechanical stress is an emergent property of the spindle positioning mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Spindle positioning and its impact on vertebrate tissue architecture and cell fate.Nature reviews. Molecular cell biology · 2021Review
- The Nuclear Mitotic Apparatus (NuMA) Protein: A Key Player for Nuclear Formation, Spindle Assembly, and Spindle Positioning.Frontiers in cell and developmental biology · 2021Review
- Abscission Couples Cell Division to Embryonic Stem Cell Fate.Developmental cell · 2020Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Proliferating animal cells are able to orient their mitotic spindles along their interphase cell axis, setting up the axis of cell division, despite rounding up as they enter mitosis. This has previously been attributed to molecular memory and, more specifically, to the maintenance of adhesions and retraction fibers in mitosis [1-6], which are thought to act as local cues that pattern cortical Gαi, LGN, and nuclear mitotic apparatus protein (NuMA) [3, 7-18]. This cortical machinery then recruits and activates Dynein motors, which pull on astral microtubules to position the mitotic spindle. Here, we reveal a dynamic two-way crosstalk between the spindle and cortical motor complexes that depends on a Ran-guanosine triphosphate (GTP) signal [12], which is sufficient to drive continuous monopolar spindle motion independently of adhesive cues in flattened human cells in culture. Building on previous work [1, 12, 19-23], we implemented a physical model of the system that recapitulates the observed spindle-cortex interactions. Strikingly, when this model was used to study spindle dynamics in cells entering mitosis, the chromatin-based signal was found to preferentially clear force generators from the short cell axis, so that cortical motors pulling on astral microtubules align bipolar spindles with the interphase long cell axis, without requiring a fixed cue or a physical memory of interphase shape. Thus, our analysis shows that the ability of chromatin to pattern the cortex during the process of mitotic rounding is sufficient to translate interphase shape into a cortical pattern that can be read by the spindle, which then guides the axis of cell division.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.