ArticlePloS one2020
Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Water limitation modifies vocal performance in California mice: implications for acoustic communication under environmental stress.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Article
- Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.Journal of speech, language, and hearing research : JSLHR · 2026Article
- Molecular changes, histopathology, and ultrasonic vocalization acoustic profiles of systemically dehydrated rats.PloS one · 2025Article
- A Review on In Vivo Research Dehydration Models and Application of Rehydration Strategies.Nutrients · 2024Review
- High-Resolution Profiling of Human Vocal Fold Cellular Landscapes With Single-Nuclei RNA Sequencing.The Laryngoscope · 2024Article
- In Vivo Visualization and Quantification of Rat Laryngeal Blood Supply After Hydration Challenge.The Laryngoscope · 2024Article
- Impact of Rehydration Following Systemic Dehydration on Vocal Fold Gene Expression.The Laryngoscope · 2023Article
- Comparative proteomic changes in rabbit vocal folds undergoing systemic dehydration and systemic rehydration.Journal of proteomics · 2023Article
- An optimized method for high-quality RNA extraction from distinctive intrinsic laryngeal muscles in the rat model.Scientific reports · 2022Article
- The role of systemic dehydration in vocal fold healing: Preliminary findings.Laryngoscope investigative otolaryngology · 2022Article
- Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration.BMC genomics · 2022Article
- Furosemide-induced systemic dehydration alters the proteome of rabbit vocal folds.Journal of proteomics · 2022Article
- Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Vocal folds are a viscoelastic multilayered structure responsible for voice production. Vocal fold epithelial damage may weaken the protection of deeper layers of lamina propria and thyroarytenoid muscle and impair voice production. Systemic dehydration can adversely affect vocal function by creating suboptimal biomechanical conditions for vocal fold vibration. However, the molecular pathobiology of systemically dehydrated vocal folds is poorly understood. We used an in vivo rabbit model to investigate the complete gene expression profile of systemically dehydrated vocal folds. The RNA-Seq based transcriptome revealed 203 differentially expressed (DE) vocal fold genes due to systemic dehydration. Interestingly, function enrichment analysis showed downregulation of genes involved in cell adhesion, cell junction, inflammation, and upregulation of genes involved in cell proliferation. RT-qPCR validation was performed for a subset of DE genes and confirmed the downregulation of DSG1, CDH3, NECTIN1, SDC1, S100A9, SPINK5, ECM1, IL1A, and IL36A genes. In addition, the upregulation of the transcription factor NR4A3 gene involved in epithelial cell proliferation was validated. Taken together, these results suggest an alteration of the vocal fold epithelial barrier independent of inflammation, which could indicate a disruption and remodeling of the epithelial barrier integrity. This transcriptome provides a first global picture of the molecular changes in vocal fold tissue in response to systemic dehydration. The alterations observed at the transcriptional level help to understand the pathobiology of dehydration in voice function and highlight the benefits of hydration in voice therapy.
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