Evidence map›Paper›PMID 32735103›Full record

ArticleJournal of veterinary science2020

A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus.

Yanqiu Wei, Baozhi Yang, Yunlong Li, Yongcheng Duan, Deyu Tian, Baoxiang He, Chuangfu Chen, Wenjun Liu, Limin Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Yanqiu Wei *CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.ORCID https://orcid.org/0000-0001-8306-041X
Baozhi Yang *College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 530004, China.ORCID https://orcid.org/0000-0003-4354-5935
Yunlong LiCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.ORCID https://orcid.org/0000-0001-6005-2150
Yongcheng DuanCollege of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 530004, China.ORCID https://orcid.org/0000-0003-0877-6447
Deyu TianCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.ORCID https://orcid.org/0000-0002-6565-3323
Baoxiang HeCollege of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 530004, China.ORCID https://orcid.org/0000-0002-2040-2651
Chuangfu ChenCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, China.ORCID https://orcid.org/0000-0002-9222-5260
Wenjun LiuCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.ORCID https://orcid.org/0000-0002-3080-8655
Limin YangCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.ORCID https://orcid.org/0000-0002-1158-0800
Chinese Academy of Sciences · CNGuangxi University · CNShihezi University · CN

Funding

National Key Program for Infectious Diseases of China 2016ZX10004222-006National Key Technologies Research and Development Program of China 2018YFC1200500National Key Technologies Research and Development Program of China 2018YFC1200600National Natural Science Foundation of China 81621091
6 · The paper itself

Abstract

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

Indexed as

AnimalsAntibodies, ViralChromatography, AffinityEnzyme-Linked Immunosorbent AssayPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusReagent StripsSwineAntibodies, ViralReagent StripsFluorescenceimmunochromatographic assayimmunochromatographypen-side detectionPRRSV

Identifiers

PMID32735103
PMCPMC7402939
OpenAlexW3042737911

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.