ArticleJournal of experimental botany2020
Generating and characterizing single- and multigene mutants of the Rubisco small subunit family in Arabidopsis.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Small subunit isoform diversity underlies structural heterogeneity in native plant Rubisco.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Proteomics and bioinformatics guided discovery of microalgal multifunctional peptides for novel nutraceutical applications.Bioprocess and biosystems engineering · 2025Article
- A Comparative Bioinformatic Investigation of the Rubisco Small Subunit Gene Family in True Grasses Reveals Novel Targets for Enhanced Photosynthetic Efficiency.International journal of molecular sciences · 2025Article
- Genetic engineering of RuBisCO by multiplex CRISPR editing small subunits in rice.Plant biotechnology journal · 2025Article
- Synthetic biology and artificial intelligence in crop improvement.Plant communications · 2025Review
- Ethylene-mediated metabolic priming increases photosynthesis and metabolism to enhance plant growth and stress tolerance.PNAS nexus · 2023Article
- Predicting plant Rubisco kinetics from RbcL sequence data using machine learning.Journal of experimental botany · 2023Article
- Temperature-induced changes in Arabidopsis Rubisco activity and isoform expression.Journal of experimental botany · 2023Article
- Red Rubiscos and opportunities for engineering green plants.Journal of experimental botany · 2023Review
- The small subunit of Rubisco and its potential as an engineering target.Journal of experimental botany · 2023Review
- Reduction in chloroplastic ribulose-5-phosphate-3-epimerase decreases photosynthetic capacity in Arabidopsis.Frontiers in plant science · 2022Article
- Big progress for small subunits: new Rubisco mutants in Arabidopsis.Journal of experimental botany · 2020Article
- CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family inFrontiers in genome editing · 2020Article
Corrections and comments
- Commented on by
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The primary CO2-fixing enzyme Rubisco limits the productivity of plants. The small subunit of Rubisco (SSU) can influence overall Rubisco levels and catalytic efficiency, and is now receiving increasing attention as a potential engineering target to improve the performance of Rubisco. However, SSUs are encoded by a family of nuclear rbcS genes in plants, which makes them challenging to engineer and study. Here we have used CRISPR/Cas9 [clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9] and T-DNA insertion lines to generate a suite of single and multiple gene knockout mutants for the four members of the rbcS family in Arabidopsis, including two novel mutants 2b3b and 1a2b3b. 1a2b3b contained very low levels of Rubisco (~3% relative to the wild-type) and is the first example of a mutant with a homogenous Rubisco pool consisting of a single SSU isoform (1B). Growth under near-outdoor levels of light demonstrated Rubisco-limited growth phenotypes for several SSU mutants and the importance of the 1A and 3B isoforms. We also identified 1a1b as a likely lethal mutation, suggesting a key contributory role for the least expressed 1B isoform during early development. The successful use of CRISPR/Cas here suggests that this is a viable approach for exploring the functional roles of SSU isoforms in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.