Evidence map›Paper›PMID 32733532›Full record

ReviewFrontiers in genetics2020

Methodologies for Transcript Profiling Using Long-Read Technologies.

Spyros Oikonomopoulos, Anthony Bayega, Somayyeh Fahiminiya, Haig Djambazian, Pierre Berube, Jiannis Ragoussis

Abstract readReview
In one paragraph

Review in Frontiers in genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed.

  1. Nanopore Sequencing for Chikungunya Virus: Principles and Application.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  2. Review
  3. Long non-coding RNAs in exercise: the hidden regulators of adaptation.Pflugers Archiv : European journal of physiology · 2026
    Review
  4. Review
  5. Integrating metagenomics and metatranscriptomics intoThe Journal of general virology · 2026
    Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Defining the True Native Ends of RNAs at Single-Molecule Level with TERA-Seq.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  20. Review

11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Spyros OikonomopoulosMcGill Genome Centre, Department of Human Genetics, McGill University, Montréal, QC, Canada.
Anthony BayegaMcGill Genome Centre, Department of Human Genetics, McGill University, Montréal, QC, Canada.
Somayyeh FahiminiyaMcGill Genome Centre, Department of Human Genetics, McGill University, Montréal, QC, Canada.
Haig DjambazianMcGill Genome Centre, Department of Human Genetics, McGill University, Montréal, QC, Canada.
Pierre BerubeMcGill Genome Centre, Department of Human Genetics, McGill University, Montréal, QC, Canada.
Jiannis RagoussisMcGill Genome Centre, Department of Human Genetics, McGill University, Montréal, QC, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA sequencing using next-generation sequencing technologies (NGS) is currently the standard approach for gene expression profiling, particularly for large-scale high-throughput studies. NGS technologies comprise high throughput, cost efficient short-read RNA-Seq, while emerging single molecule, long-read RNA-Seq technologies have enabled new approaches to study the transcriptome and its function. The emerging single molecule, long-read technologies are currently commercially available by Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), while new methodologies based on short-read sequencing approaches are also being developed in order to provide long range single molecule level information-for example, the ones represented by the 10x Genomics linked read methodology. The shift toward long-read sequencing technologies for transcriptome characterization is based on current increases in throughput and decreases in cost, making these attractive for

Indexed as

long readnanoporenext-generation sequencingPacBioRNA-Seqtranscriptome

Identifiers

PMID32733532
PMCPMC7358353

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.