Evidence map›Paper›PMID 32732422›Full record

ArticleScience (New York, N.Y.)2020

In-cell architecture of an actively transcribing-translating expressome.

Francis J O'Reilly, Liang Xue, Andrea Graziadei, Ludwig Sinn, Swantje Lenz, Dimitry Tegunov, Cedric Blötz, Neil Singh, Wim J H Hagen, Patrick Cramer and 3 more

Open access · greenAbstract read
In one paragraph

Article in Science (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 142 papers.

0numbers the graph read from it
0cells of the map it votes in
142citing papers in PubMed
15.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

142 citing papers in PubMed, 282 citations in OpenAlex.

  1. The phenotypic landscape of the model firmicuteProceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Structural basis of long-range transcription-translation coupling.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Review
  17. HflX mediates rifampicin resistance inFrontiers in microbiology · 2026
    Article
  18. Article
  19. Article
  20. Article

82 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 2 countries.

Francis J O'Reilly *Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.ORCID 0000-0001-9258-0150
Liang Xue *Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.ORCID 0000-0003-4368-2526
Andrea Graziadei *Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.ORCID 0000-0001-7709-6002
Ludwig SinnBioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.ORCID 0000-0003-4692-0681
Swantje LenzBioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.ORCID 0000-0002-8839-5371
Dimitry TegunovDepartment of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany.ORCID 0000-0001-7019-3221
Cedric BlötzDepartment of General Microbiology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany.
Neil SinghDepartment of General Microbiology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany.ORCID 0000-0001-7256-003X
Wim J H HagenStructural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.ORCID 0000-0001-6229-2692
Patrick CramerDepartment of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany.ORCID 0000-0001-5454-7755
Jörg StülkeDepartment of General Microbiology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany.ORCID 0000-0001-5881-5390
Julia MahamidStructural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany. julia.mahamid@embl.de juri.rappsilber@tu-berlin.de.ORCID 0000-0001-6968-041X
Juri RappsilberBioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany. julia.mahamid@embl.de juri.rappsilber@tu-berlin.de.ORCID 0000-0001-5999-1310
Technische Universität Berlin · DEUniversity of Göttingen · DEEuropean Molecular Biology Laboratory · DEMax Planck Institute for Biophysical Chemistry · DEHeidelberg University · DEWellcome Centre for Cell Biology · GB

Funding

Deutsche ForschungsgemeinsEuropean Research Council 693023European Research Council 760067Wellcome Trust 103139Wellcome Trust 203149
6 · The paper itself

Abstract

Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen

Indexed as

Peptide Chain Elongation, TranslationalProtein Interaction MapsTranscription, GeneticBacterial ProteinsDNA-Directed RNA PolymerasesGenome, BacterialHumansMycoplasma pneumoniaePeptide Elongation FactorsRibosomesTranscriptomeBacterial ProteinsDNA-Directed RNA PolymerasesPeptide Elongation Factors

Identifiers

PMID32732422
PMCPMC7115962
OpenAlexW3046695191

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.