Evidence map›Paper›PMID 32732289›Full record

ArticleThe Journal of biological chemistry2020

Gravin-associated kinase signaling networks coordinate γ-tubulin organization at mitotic spindle poles.

Paula J Bucko, Irvin Garcia, Ridhima Manocha, Akansha Bhat, Linda Wordeman, John D Scott

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Paula J BuckoDepartment of Pharmacology, University of Washington, Seattle, Washington, USA.
Irvin GarciaDepartment of Pharmacology, University of Washington, Seattle, Washington, USA.
Ridhima ManochaDepartment of Pharmacology, University of Washington, Seattle, Washington, USA.
Akansha BhatDepartment of Pharmacology, University of Washington, Seattle, Washington, USA.
Linda WordemanDepartment of Physiology and Biophysics, University of Washington, Seattle, Washington, USA.
John D ScottDepartment of Pharmacology, University of Washington, Seattle, Washington, USA. Electronic address: scottjdw@uw.edu.
University of Washington · US

Funding

Microtubule Dynamics and Chromosome SegregationR01GM069429 · NIGMS · UNIVERSITY OF WASHINGTON · PI WORDEMAN, LINDA · 2004 to 2020
$5.6M
Defective PKA Signaling in Cushing's SyndromeR01DK119192 · NIDDK · UNIVERSITY OF WASHINGTON · PI John D Scott · 2018 to 2026
$4.4M
AKAP Modulation of Renal SignalingR01DK119186 · NIDDK · UNIVERSITY OF WASHINGTON · PI SCOTT, JOHN D · 2019 to 2022
$1.5M
NIDDK NIH HHS R01 DK119186NIDDK NIH HHS R01 DK119192NIGMS NIH HHS R01 GM069429
6 · The paper itself

Abstract

Mitogenic signals that regulate cell division often proceed through multienzyme assemblies within defined intracellular compartments. The anchoring protein Gravin restricts the action of mitotic kinases and cell-cycle effectors to defined mitotic structures. In this report we discover that genetic deletion of Gravin disrupts proper accumulation and asymmetric distribution of γ-tubulin during mitosis. We utilize a new precision pharmacology tool, Local Kinase Inhibition, to inhibit the Gravin binding partner polo-like kinase 1 at spindle poles. Using a combination of gene-editing approaches, quantitative imaging, and biochemical assays, we provide evidence that disruption of local polo-like kinase 1 signaling underlies the γ-tubulin distribution defects observed with Gravin loss. Our study uncovers a new role for Gravin in coordinating γ-tubulin recruitment during mitosis and illuminates the mechanism by which signaling enzymes regulate this process at a distinct subcellular location.

Indexed as

Signal TransductionA Kinase Anchor ProteinsCell Cycle ProteinsHEK293 CellsHeLa CellsHumansPolo-Like Kinase 1Protein Serine-Threonine KinasesProto-Oncogene ProteinsSpindle ApparatusTubulinAKAP12 protein, humanA Kinase Anchor ProteinsCell Cycle ProteinsPolo-Like Kinase 1Protein Serine-Threonine KinasesProto-Oncogene ProteinsTUBG1 protein, humanTubulinenzyme inhibitormitosisprotein complexprotein kinaseprotein kinase A anchoring protein (AKAP)tubulin

Identifiers

PMID32732289
PMCPMC7535905
OpenAlexW3046346734

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.