ArticleScience (New York, N.Y.)2020
Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy.
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Article in Science (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 534 papers, 1 of them a synthesis that pooled it.
0numbers the graph read from it
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534citing papers in PubMed, 1 pooled it
18.6field-weighted citation impact, top 1% of its field
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Who cites it
534 citing papers in PubMed, 1 synthesis or guideline pooled it, 835 citations in OpenAlex.
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- Triple IFN pathway deficiency sensitizes mice to human respiratory virus infection independent of human viral receptor expression.Nature communications · 2026Article
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- A biosafe mouse model for SARS-CoV-2 infection that more realistically simulates COVID-19 symptoms.Signal transduction and targeted therapy · 2026Article
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- IFITM3 deficiency drives SARS-CoV-2 adaptation while preserving variant-specific traits.Nature communications · 2026Article
- SARS-CoV-2 variants: biology, pathogenicity, immunity and control.Nature reviews. Microbiology · 2026Review
- Dynamics of lung-infiltrating virus-specific T cells associated with age-dependent SARS-CoV-2 pneumonia severity.PLoS pathogens · 2026Article
- Experimental evolution of Rc-o319 sarbecovirus spike protein reveals limited ACE2 adaptability.Virus evolution · 2026Article
- A binary self-amplifying expression platform enabling lipid nanoparticle-free vaccines and nanomedicines.Nature communications · 2025Article
- Monocyte-eosinophil signaling axis promotes vaccine-mediated protection against SARS-CoV-2.PLoS pathogens · 2025Article
474 more citing papers are in PubMed but not listed here.
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Authors and funding
32 authors at 5 institutions in 2 countries.
Hongjing Gu *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-6701-5798
Qi Chen *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-6568-7267
Guan Yang *State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China.ORCID 0000-0002-4026-6976
Lei He *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0001-6955-0121
Hang Fan *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-7927-1684
Yong-Qiang Deng *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-2306-1039
Yanxiao WangState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China.
Yue TengState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Zhongpeng ZhaoState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-9147-6252
Yujun CuiState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0001-7236-2412
Yuchang LiState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Xiao-Feng LiState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-5014-7102
Jiangfan LiState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0001-6884-104X
Na-Na ZhangState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0003-4669-7897
Xiaolan YangState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-6903-5995
Shaolong ChenState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Yan GuoState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Guangyu ZhaoState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-0925-5216
Xiliang WangState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0001-6128-1342
De-Yan LuoState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Hui WangState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Xiao YangState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China.ORCID 0000-0002-0298-9831
Yan LiInstitute of Military Cognition and Brain Sciences, Beijing 100850, China.
Gencheng HanInstitute of Military Cognition and Brain Sciences, Beijing 100850, China.
Yuxian HeInstitute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Xiaojun ZhouLaboratory Animal Center, Academy of Military Medical Sciences, Beijing 100071, China.
Shusheng GengBeijing JOINN Biologics Co., Beijing 100176, China.
Xiaoli ShengBeijing JOINN Biologics Co., Beijing 100176, China.
Shibo Jiang *Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Fudan University, Shanghai 200032, China. shibojiang@fudan.edu.cn sunsh01@163.com qincf@bmi.ac.cn.ORCID 0000-0001-8283-7135
Shihui Sun *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. shibojiang@fudan.edu.cn sunsh01@163.com qincf@bmi.ac.cn.ORCID 0000-0002-2623-9939
Cheng-Feng Qin *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. shibojiang@fudan.edu.cn sunsh01@163.com qincf@bmi.ac.cn.ORCID 0000-0002-0632-2807
Yusen Zhou *State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.ORCID 0000-0002-7301-4089
Academy of Military Medical Sciences · CNBeijing Proteome Research Center · CNUS Biologic (United States) · USChinese Academy of Medical Sciences & Peking Union Medical College · CNFudan University · CN
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the respiratory tract of aged BALB/c mice. The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein. The protective efficacy of a recombinant RBD vaccine candidate was validated by using this model. Thus, this mouse-adapted strain and associated challenge model should be of value in evaluating vaccines and antivirals against SARS-CoV-2.
Indexed as
Disease Models, AnimalMiceAdministration, IntranasalAngiotensin-Converting Enzyme 2AnimalsBetacoronavirusCoronavirus InfectionsCOVID-19COVID-19 VaccinesFemaleHigh-Throughput Nucleotide SequencingHumansImmunogenicity, VaccineLungLung Diseases, InterstitialMice, Inbred BALB CACE2 protein, humanAce2 protein, mouseAngiotensin-Converting Enzyme 2COVID-19 VaccinesPeptidyl-Dipeptidase ASpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Vaccines, SyntheticViral Vaccines
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