Evidence map›Paper›PMID 32731875›Full record

ArticleVirology journal2020

Association of genotype III of dengue virus serotype 3 with disease outbreak in Eastern Sudan, 2019.

Mawahib H Eldigail, Hazem A Abubaker, Fatima A Khalid, Tajeldin M Abdallah, Hassan H Musa, Mohamed E Ahmed, Gamal K Adam, Mustafa I Elbashir, Imadeldin E Aradaib

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Mawahib H EldigailMolecular Biology Laboratory, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32, Khartoum North, Sudan.
Hazem A AbubakerMolecular Biology Laboratory, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32, Khartoum North, Sudan.
Fatima A KhalidDepartment of Internal Medicine and Microbiology, Faculty of Medicine, University of Kassala, El-Gadarif, Sudan.
Tajeldin M AbdallahDepartment of Internal Medicine and Microbiology, Faculty of Medicine, University of Kassala, El-Gadarif, Sudan.
Hassan H MusaDepartment of Medical Microbiology, Faculty of Medical laboratory Sciences, University of Khartoum, El-Gadarif, Sudan.
Mohamed E AhmedZamzam Unit for Medical Research (ZUMR), Vectore Borne and Zoonotic Diseases Research Laboratory, Zamzam University College, Khartoum, Sudan.
Gamal K AdamDepartment of Microbiology, Faculty of Medicine, University of El-Gadarif, El-Gadarif, Sudan.
Mustafa I ElbashirDepartment of Microbiology, Faculty of Medicine, University of Khartoum, El-Gadarif, Sudan.
Imadeldin E AradaibMolecular Biology Laboratory, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32, Khartoum North, Sudan. aradaib@yahoo.com.
University of Khartoum · SDKassala University · SDGadarif University · SD

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDengue fever (DF) is an arthropod-borne disease caused by dengue virus (DENV). DENV is a member of the genus Flavivirus in the family Flaviviridae. Recently, DENV has been reported as an important emerging infectious viral pathogen in Sudan. Multiple outbreaks and sporadic cases of DF have been frequently reported in the eastern region of Sudan. The present study was conducted to confirm DENV outbreak in Kassala State, eastern Sudan, 2019, and to provide some information on the molecular characterization of the DENV isolate associated with the disease outbreak.

methodsA hundred serum samples were collected during the outbreak from residents of Kassala State, Sudan, 2019. ELISA was used to detect DENV non structural protein NS1 (DENV-NS1) in acute phase sera sampled during the disease outbreak. RT-PCR assays were used to amplify a fragment of the capsid/pre-membrane region (CprM) of the viral polyprotein gene. The PCR products of the amplified CprM region of the viral polyprotein gene were purified and partial sequences were generated and used to confirm the specificity of DENV sequences and to identify the virus serotype. Phylogenetic tree was constructed to determine the genotype of DENV associated with the outbreak.

resultsUsing DENV-NS1 ELISA assay, DENV infection was confirmed in 23% sampled sera. The detection of DENV RNA was made possible using group-specific RT-PCR assay. The virus was serotyped as DENV serotype 3 (DENV-3) using DENV serotype-specific RT-PCR assay. Phylogenetic analysis of the partial CprM sequences of the viral polyprotein gene indicates that the virus belonged to genotype III of DENV-3.

conclusionThe scientific data presented in this investigation confirmed that genotype III of DENV-3 was associated with the disease outbreak in eastern Sudan, 2019. The study represents the first report on molecular characterization of DENV-3 in Sudan.

Indexed as

Disease OutbreaksPhylogenyDengueDengue VirusGenotypeHumansSequence Analysis, DNASerogroupSudanDengue virusELISAPhylogenyRT-PCR; sequence analysisSudan

Identifiers

PMID32731875
PMCPMC7392696
OpenAlexW3045615675

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.