Evidence map›Paper›PMID 32731804›Full record

ArticleTissue engineering. Part A2021

Matrix Stiffness Modulates Patient-Derived Glioblastoma Cell Fates in Three-Dimensional Hydrogels.

Christine Wang, Sauradeep Sinha, Xinyi Jiang, Luke Murphy, Sergio Fitch, Christy Wilson, Gerald Grant, Fan Yang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part A, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
4.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 76 citations in OpenAlex.

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  17. Exploiting Matrix Stiffness to Overcome Drug Resistance.ACS biomaterials science & engineering · 2024
    Review
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  19. RSC advances · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Christine WangDepartment of Bioengineering, Schools of Engineering and Medicine, Stanford University, Stanford, California, USA.
Sauradeep SinhaDepartment of Bioengineering, Schools of Engineering and Medicine, Stanford University, Stanford, California, USA.
Xinyi JiangDepartment of Orthopaedic Surgery, Stanford University, Stanford, California, USA.
Luke MurphyDepartment of Bioengineering, Schools of Engineering and Medicine, Stanford University, Stanford, California, USA.
Sergio FitchDepartment of Orthopaedic Surgery, Stanford University, Stanford, California, USA.
Christy WilsonDepartment of Neurosurgery, Stanford University, School of Medicine, Stanford, California, USA.
Gerald GrantDepartment of Neurosurgery, Stanford University, School of Medicine, Stanford, California, USA.
Fan YangDepartment of Bioengineering, Schools of Engineering and Medicine, Stanford University, Stanford, California, USA.
Stanford University · US

Funding

Graduate Training Program in BiotechnologyT32GM008412 · NIGMS · STANFORD UNIVERSITY · PI COCHRAN, JENNIFER R · 1991 to 2020
$5.5M
Engineering Brain Cancer in a Dish: Hydrogel-based 3D in vitro Models for Pediatric Brain TumorF31CA246972 · NCI · STANFORD UNIVERSITY · PI SINHA, SAURADEEP · 2020 to 2023
$126k
NCI NIH HHS F31 CA246972NIGMS NIH HHS T32 GM008412
6 · The paper itself

Abstract

Cancer progression is known to be accompanied by changes in tissue stiffness. Previous studies have primarily employed immortalized cell lines and 2D hydrogel substrates, which do not recapitulate the 3D tumor niche. How matrix stiffness affects patient-derived cancer cell fate in 3D remains unclear. In this study, we report a matrix metalloproteinase-degradable poly(ethylene-glycol)-based hydrogel platform with brain-mimicking biochemical cues and tunable stiffness (40-26,600 Pa) for 3D culture of patient-derived glioblastoma xenograft (PDTX GBM) cells. Our results demonstrate that decreasing hydrogel stiffness enhanced PDTX GBM cell proliferation, and hydrogels with stiffness 240 Pa and below supported robust PDTX GBM cell spreading in 3D. PDTX GBM cells encapsulated in hydrogels demonstrated higher drug resistance than 2D control, and increasing hydrogel stiffness further enhanced drug resistance. Such 3D hydrogel platforms may provide a valuable tool for mechanistic studies of the role of niche cues in modulating cancer progression for different cancer types.

Indexed as

Brain NeoplasmsGlioblastomaCell DifferentiationCell Line, TumorCell ProliferationHumansHydrogelsHydrogelsglioblastomahydrogelsin vitro cancer modelspatient-derived cellsstiffness

Identifiers

PMID32731804
PMCPMC7984937
OpenAlexW3046607890

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.