Evidence map›Paper›PMID 32731642›Full record

ArticleViruses2020

Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples.

Ayushi Rai, Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth A Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel V Borca, Douglas P Gladue

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
8.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 48 citations in OpenAlex.

  1. Review
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  6. Aloperine Inhibits ASFV via Regulating PRLR/JAK2 Signaling Pathway In Vitro.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Ayushi RaiARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Sarah PruittARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Elizabeth Ramirez-MedinaARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Elizabeth A VuonoARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Ediane SilvaARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Lauro Velazquez-SalinasARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Consuelo CarrilloAPHIS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Manuel V BorcaARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Douglas P GladueARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.ORCID 0000-0002-7894-0233
Oak Ridge Associated Universities · USPlum Island Animal Disease Center · USKansas State University · USAnimal and Plant Health Inspection Service · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly spread to all provinces in China and to other countries in southeast Asia, and most recently to India. Outbreaks of the disease occur in Europe as far west as Poland, and one isolated outbreak has been reported in Belgium. The current outbreak strain is highly contagious and can cause a high degree of lethality in domestic pigs, leading to widespread and costly losses to the industry. Currently, detection of infectious ASFV in field clinical samples requires accessibility to primary swine macrophage cultures, which are infrequently available in most regional veterinary diagnostic laboratories. Here, we report the identification of a commercially available cell line, MA-104, as a suitable substrate for virus isolation of African swine fever virus.

Indexed as

African Swine FeverAfrican Swine Fever VirusAnimalsCell Culture TechniquesCell LineChlorocebus aethiopsDisease OutbreaksEpithelial CellsMacrophagesSus scrofaSwineASFASFVdiagnostics

Identifiers

PMID32731642
PMCPMC7472077
OpenAlexW3045534129

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.