Evidence map›Paper›PMID 32731549›Full record

ReviewMolecules (Basel, Switzerland)2020

The Secret Lives of Fluorescent Membrane Probes as Revealed by Molecular Dynamics Simulations.

Hugo A L Filipe, Maria João Moreno, Luís M S Loura

Open access · goldAbstract readReview
In one paragraph

Review in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Growth ofbioRxiv : the preprint server for biology · 2024
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Fluorescent ProbesMolecules (Basel, Switzerland) · 2023
    Article
  12. Review
  13. Article
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Hugo A L FilipeChemistry Department, Coimbra Chemistry Center, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0001-6935-4307
Maria João MorenoCoimbra Chemistry Center and CNC-Center for Neuroscience and Cell Biology, Chemistry Department, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0003-3076-9905
Luís M S LouraCoimbra Chemistry Center and CNC-Center for Neuroscience and Cell Biology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.ORCID 0000-0002-1051-2312
University of Coimbra · PT

Funding

Fundação para a Ciência e a Tecnologia CENTRO-01-0247-FEDER-033838Fundação para a Ciência e a Tecnologia POCI-01-0247-FEDER-033368Fundação para a Ciência e a Tecnologia PT2020-PTDC/DTP-FTO/2784/2014Fundação para a Ciência e a Tecnologia UIDB/00313/2020
6 · The paper itself

Abstract

Fluorescent probes have been employed for more than half a century to study the structure and dynamics of model and biological membranes, using spectroscopic and/or microscopic experimental approaches. While their utilization has led to tremendous progress in our knowledge of membrane biophysics and physiology, in some respects the behavior of bilayer-inserted membrane probes has long remained inscrutable. The location, orientation and interaction of fluorophores with lipid and/or water molecules are often not well known, and they are crucial for understanding what the probe is actually reporting. Moreover, because the probe is an extraneous inclusion, it may perturb the properties of the host membrane system, altering the very properties it is supposed to measure. For these reasons, the need for independent methodologies to assess the behavior of bilayer-inserted fluorescence probes has been recognized for a long time. Because of recent improvements in computational tools, molecular dynamics (MD) simulations have become a popular means of obtaining this important information. The present review addresses MD studies of all major classes of fluorescent membrane probes, focusing in the period between 2011 and 2020, during which such work has undergone a dramatic surge in both the number of studies and the variety of probes and properties accessed.

Indexed as

Molecular Dynamics SimulationFluorescent DyesLipid BilayersFluorescent DyesLipid Bilayersfluorescencelipid bilayermembrane probesmolecular dynamicsmolecular simulation

Identifiers

PMID32731549
PMCPMC7435664
OpenAlexW3046142679

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.