2-Ketoglutarate-Generated In Vitro Enzymatic Biosystem Facilitates Fe(II)/2-Ketoglutarate-Dependent Dioxygenase-Mediated C-H Bond Oxidation for (2

Xiao-Ran Jing, Huan Liu, Yao Nie, Yan Xu

Open access · goldAbstract read

In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

0.6field-weighted citation impact, top 37% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

Efficient and scalable synthesis of 1,5-diamino-2-hydroxy-pentane from L-lysine via cascade catalysis using engineered Escherichia coli.Microbial cell factories · 2022
Article
Designing of an Efficient Whole-Cell Biocatalyst System for Converting L-Lysine Into Cis-3-Hydroxypipecolic Acid.Frontiers in microbiology · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Xiao-Ran JingKey Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.

Huan LiuKey Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.

Yao NieKey Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.ORCID 0000-0001-8065-7640

Yan XuKey Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.

Jiangnan University · CN

Funding

111 Project 111-2-06Fundamental Research Funds for the Central Universities JUSRP51504High-End Foreign Experts Recruitment Program G20190010083National First-Class Discipline Program of Light Industry Technology and Engineering LITE2018-09National Natural Science Foundation of China No. 21676120Postgraduate Research & Practice Innovation Program of Jiangsu Province KYCX19_1833Program for Advanced Talents within Six Industries of Jiangsu Province 2015-NY-007Program for the Key Laboratory of Enzymes of Suqian M201803

6 · The paper itself

Abstract

Fe(II)/2-ketoglutarate-dependent dioxygenase (Fe(II)/2-KG DO)-mediated hydroxylation is a critical type of C-H bond functionalization for synthesizing hydroxy amino acids used as pharmaceutical raw materials and precursors. However, DO activity requires 2-ketoglutarate (2-KG), lack of which reduces the efficiency of Fe(II)/2-KG DO-mediated hydroxylation. Here, we conducted multi-enzymatic syntheses of hydroxy amino acids. Using (2

Indexed as

Amino Acid OxidoreductasesCatalaseCell-Free SystemDioxygenasesHydrogen PeroxideIronIsoleucineKetoglutaric Acids4-hydroxyisoleucineAmino Acid OxidoreductasesCatalaseDioxygenasesHydrogen PeroxideIronIsoleucineKetoglutaric AcidsL-glutamate oxidase2-ketoglutarate generationFe(II)/2-ketoglutarate-dependent dioxygenasehydroxy amino acidsregio- and stereo-selective synthesissequential cascade reaction

Identifiers

PMID32731373

DOI10.3390/ijms21155347

PMCPMC7432852

OpenAlexW3045539769

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.