ArticlePloS one2020
Pooled RNA sample reverse transcriptase real time PCR assay for SARS CoV-2 infection: A reliable, faster and economical method.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Diagnostic value comparative analysis of the commercial kits for the detection of SARS-CoV-2 in clinical samples: a systematic review and meta-analysis.Iranian journal of microbiology · 2025Review
- Optimizing surveillance: feasibility of pooled sample testing for bovine alphaherpesvirus 1 detection by real-time PCR.Virusdisease · 2025Article
- Oropouche Virus Importation in Southern Brazil and Emerging Concern Calling for Enhanced Public Health Surveillance.Journal of medical virology · 2025Article
- Opportunity for severe and critical COVID-19 pneumonia treatment with corticosteroids: a retrospective cohort study.Journal of thoracic disease · 2024Article
- A proactive/reactive mass screening approach with uncertain symptomatic cases.PLoS computational biology · 2024Article
- Assuring safety of fecal microbiota transplantation in the COVID-19 era: A single-center experience.JGH open : an open access journal of gastroenterology and hepatology · 2023Article
- A novel strategy to avoid sensitivity loss in pooled testing for SARS-CoV-2 surveillance: validation using nasopharyngeal swab and saliva samples.Frontiers in public health · 2023Article
- Real-world Evaluation of a Sample Pooling Strategy for Large-Scale Rapid COVID-19 Testing.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2022Article
- Detection of SARS-CoV-2 RNA in Upper Respiratory Swap Samples by Pooling MethodBalkan medical journal · 2022Article
- Article
- SARS-CoV-2 Diagnostics Based on Nucleic Acids Amplification: From Fundamental Concepts to Applications and Beyond.Frontiers in cellular and infection microbiology · 2022Review
- Practical considerations to establish a validated platform for pooled detection of SARS-CoV-2 by droplet digital PCR.PloS one · 2022Article
- Article
- Pooled Specimen Testing Using Automated Cartridge-Based System for COVID-19: The Cost on Sensitivity.The Malaysian journal of medical sciences : MJMS · 2021Article
- Pooled Testing Strategies for SARS-CoV-2 diagnosis: A comprehensive review.Diagnostic microbiology and infectious disease · 2021Review
- The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving reagents and improving turnaround time.Journal of clinical laboratory analysis · 2021Article
- Efficiency of pooled surveillance testing in academic labs to detect and inhibit COVID-19 outbreaks.Bioanalysis · 2021Article
- Misdiagnosis of SARS-CoV-2: A Critical Review of the Influence of Sampling and Clinical Detection Methods.Medical sciences (Basel, Switzerland) · 2021Review
- Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests.Science translational medicine · 2021Article
- Pooled nasopharyngeal swab collection in a single vial for the diagnosis of SARS CoV-2 infection: An effective cost saving method.Indian journal of medical microbiology · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCorona virus disease 2019 (COVID-19) which initially started as a cluster of pneumonia cases in the Wuhan city of China has now become a full-blown pandemic. Timely diagnosis of COVID-19 is the key in containing the pandemic and breaking the chain of transmission. In low- and middle-income countries availability of testing kits has become the major bottleneck in testing. Novel methods like pooling of samples are the need of the hour.
objectiveWe undertook this study to evaluate a novel protocol of pooling of RNA samples/elutes in performance of PCR for SARS CoV-2 virus. STUDY
designExtracted RNA samples were randomly placed in pools of 8 on a 96 well plate. Both individual RNA (ID) and pooled RNA RT-qPCR for the screening E gene were done in the same plate and the positivity for the E gene was seen.
resultsThe present study demonstrated that pool testing with RNA samples can easily detect even up to a single positive sample with Ct value as high as 38. The present study also showed that the results of pool testing is not affected by number of positive samples in a pool.
conclusionPooling of RNA samples can reduce the time and expense, and can help expand diagnostic capabilities, especially during constrained supply of reagents and PCR kits for the diagnosis of SARS-CoV-2 infection.
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