Evidence map›Paper›PMID 32730339›Full record

ArticlePLoS neglected tropical diseases2020

A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method.

Vivian Favero, Carolina De Marco Veríssimo, Angela R Piovesan, Alessandra L Morassutti, André A Souto, Hélio R Bittencourt, Vanessa F Pascoal, Catieli G Lindholz, Malcolm K Jones, Renata P Souza and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 3 countries.

Vivian FaveroResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.ORCID 0000-0002-5145-4606
Carolina De Marco VeríssimoResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Angela R PiovesanNeurotoxins Laboratory (LaNeuroTox), School of Medicine, Brain Institute and School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Alessandra L MorassuttiResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
André A SoutoPolytechnic School, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Hélio R BittencourtPolytechnic School, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Vanessa F PascoalResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Catieli G LindholzResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Malcolm K JonesSchool of Veterinary Science, The University of Queensland, Brisbane, Qld, Australia, 4072.ORCID 0000-0002-0035-6141
Renata P SouzaResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Francine De Vargas RigoResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Célia R CarliniNeurotoxins Laboratory (LaNeuroTox), School of Medicine, Brain Institute and School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Carlos Graeff-TeixeiraResearch Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
Pontifícia Universidade Católica do Rio Grande do Sul · BROllscoil na Gaillimhe – University of Galway · IEThe University of Queensland · AUUniversidade Federal do Rio Grande do Sul · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Schistosomiasis remains a serious public health problem in tropical regions, affecting more than 250 million people. Sensitive diagnostic methods represent key tools for disease elimination, in particular in areas with low endemicity. Advances in the use of luminol-based chemiluminescent techniques have enabled greater sensitivity and speed in obtaining results in different diagnostic settings. In this study, we developed a luminol-H2O2 chemiluminescence (CL) method to detect Schistosoma mansoni eggs in human fecal sediments processed by the Helmintex (HTX) method. After S. mansoni eggs were incubated with a solution of luminol-H2O2 the light emission was detected and measured by spectrophotometry at 431 nm for 5 min, using detection and counts of eggs by bright field optical microscopy as a reference. CL intensity was found to correlate with different sources and numbers of eggs. Furthermore, our results showed that the CL method can distinguish positive from negative samples with 100% sensitivity and 71% specificity. To our knowledge, this is the first study to report the use of CL for the diagnosis of helminths from fecal samples. The combination of the HTX method with CL represents an important advance in providing a reference method with the highest standards of sensitivity.

Indexed as

OvumAnimalsFecesHumansHydrogen PeroxideLuminescent MeasurementsLuminolMiceSchistosoma mansoniSchistosomiasis mansoniHydrogen PeroxideLuminol

Identifiers

PMID32730339
PMCPMC7437924
OpenAlexW3046781811

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.