ArticlePLoS biology2020
In situ characterisation and manipulation of biological systems with Chi.Bio.
Article in PLoS biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Cross-feeding enables robust coexistence between four bacterial species.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Closed-Loop Optogenetic Control in a Microplate Reader.ACS synthetic biology · 2026Article
- Toxic and useful: harnessing yeast killer toxins for product recovery.Microbial cell factories · 2026Article
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- Optimized Quantitative Bacterial Two-Hybrid (qB2H) for Protein-Protein Interaction Assessment.Computational and structural biotechnology journal · 2026Article
- Article
- A stationary phase-specific bacterial green light sensor for enhancing metabolite production.Nature communications · 2025Article
- In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.Nucleic acids research · 2025Article
- A Safe and Versatile Minicell Platform Derived fromJournal of microbiology and biotechnology · 2025Article
- The Escherichia coli replication initiator DnaA is titrated on the chromosome.Nature communications · 2025Article
- Isopropanol production from carbon dioxide byiScience · 2025Article
- Engineering controllable alteration of malonyl-CoA levels to enhance polyketide production.Nature chemical biology · 2025Article
- Control of avocado anthracnose by carposphere-associated Kosakonia cowanii VG1 for agricultural applications.AMB Express · 2025Article
- Growth-coupled continuous directed evolution by MutaT7 enables efficient and automated enzyme engineering.Applied and environmental microbiology · 2025Article
- Incoherent feedback from coupled amino acids and ribosome pools generates damped oscillations in growing E. coli.Nature communications · 2025Article
- Gene syntaxes modulate gene expression and circuit behavior on plasmids.Journal of biological engineering · 2025Article
- Directing microbial co-culture composition using cybernetic control.Cell reports methods · 2025Article
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- Biotechnology systems engineering: preparing the next generation of bioengineers.Frontiers in systems biology · 2025Article
- Gain efficiency with streamlined and automated data processing: Examples from high-throughput monoclonal antibody production.PloS one · 2025Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precision and repeatability of in vivo biological studies is predicated upon methods for isolating a targeted subsystem from external sources of noise and variability. However, in many experimental frameworks, this is made challenging by nonstatic environments during host cell growth, as well as variability introduced by manual sampling and measurement protocols. To address these challenges, we developed Chi.Bio, a parallelised open-source platform that represents a new experimental paradigm in which all measurement and control actions can be applied to a bulk culture in situ. In addition to continuous-culturing capabilities, it incorporates tunable light outputs, spectrometry, and advanced automation features. We demonstrate its application to studies of cell growth and biofilm formation, automated in silico control of optogenetic systems, and readout of multiple orthogonal fluorescent proteins in situ. By integrating precise measurement and actuation hardware into a single low-cost platform, Chi.Bio facilitates novel experimental methods for synthetic, systems, and evolutionary biology and broadens access to cutting-edge research capabilities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.