Evidence map›Paper›PMID 32729511›Full record

ArticleBiosensors & bioelectronics2020

Force-dependent trans-endocytosis by breast cancer cells depletes costimulatory receptor CD80 and attenuates T cell activation.

Seungman Park, Yu Shi, Byoung Choul Kim, Myung Hyun Jo, Leilani O Cruz, Zheming Gou, Taekjip Ha, Li-Fan Lu, Daniel H Reich, Yun Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
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  5. Article
  6. The Biological Significance of Trogocytosis.Results and problems in cell differentiation · 2024
    Article
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  8. Article
  9. Article
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  11. Article
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  15. Recent developments in DNA-based mechanical nanodevices.Chemical communications (Cambridge, England) · 2022
    Review
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Seungman ParkDepartment of Mechanical Engineering, Johns Hopkins University, MD, 21218, USA; Center for Cell Dynamics, Johns Hopkins University, MD, 21218, USA; Institute for NanoBioTechnology, Johns Hopkins University, MD, 21218, USA.
Yu ShiDepartment of Physics & Astronomy, Johns Hopkins University, MD, 21218, USA.
Byoung Choul KimDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; Howard Hughes Medical Institute, Baltimore, MD, 21205, USA; Division of Nano-Bioengineering, Incheon National University, Incheon, 22012, South Korea.
Myung Hyun JoDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
Leilani O CruzDivision of Biological Science, University of California, San Diego, CA, 92093, USA.
Zheming GouDepartment of Mechanical Engineering, Johns Hopkins University, MD, 21218, USA.
Taekjip HaDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; Howard Hughes Medical Institute, Baltimore, MD, 21205, USA.
Li-Fan LuDivision of Biological Science, University of California, San Diego, CA, 92093, USA.
Daniel H ReichDepartment of Physics & Astronomy, Johns Hopkins University, MD, 21218, USA.
Yun ChenDepartment of Mechanical Engineering, Johns Hopkins University, MD, 21218, USA; Center for Cell Dynamics, Johns Hopkins University, MD, 21218, USA; Institute for NanoBioTechnology, Johns Hopkins University, MD, 21218, USA. Electronic address: yun.chen@jhu.edu.
Johns Hopkins University · USUniversity of California, San Diego · USIncheon National University · KR

Funding

Translational Aging Research Training ProgramT32AG058527 · NIA · JOHNS HOPKINS UNIVERSITY · PI Peter M. Abadir, Esther Seunghee Oh · 2018 to 2026
$4.0M
Tension-Sensitive Drug Release System to Enhance Targeting SelectivityR21EB029677 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI CHEN, YUN · 2020 to 2020
$610k
NIA NIH HHS T32 AG058527NIBIB NIH HHS R21 EB029677
6 · The paper itself

Abstract

In this study, we investigated the biophysical interaction between cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and CD80. CTLA-4 is a key molecule in immunosuppression, and CD80 is a costimulatory receptor promoting T cell activation. We observed that after cell-cell contact was established between breast cancer cells and antigen presenting cells (APCs), CTLA-4 expressed on the breast cancer cells bind to CD80 expressed on the APCs, and underwent trans-endocytosis to deplete CD80. Force measurement and live cell imaging revealed that upon binding to CD80, forces generated by breast cancer cells and transmitted via CTLA-4 were sufficiently strong to displace CD80 from the surface of APCs to be internalized by breast cancer cells. We further demonstrated that because of the force-dependent trans-endocytosis of CD80, the capacity of APCs to activate T cells was significantly attenuated. Furthermore, inhibiting force generation in cancer cells would increase the T cell activating capacity of APCs. Our results provide a possible mechanism behind the immunosuppression commonly seen in breast cancer patients, and may lead to a new strategy to restore anti-tumor immunity by inhibiting pathways of force-generation.

Indexed as

Biosensing TechniquesBreast NeoplasmsB7-2 AntigenCD28 AntigensEndocytosisHumansLymphocyte ActivationT-LymphocytesB7-2 AntigenCD28 Antigens

Identifiers

PMID32729511
PMCPMC8011575
OpenAlexW3034302834

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.