ArticleMicrobial biotechnology2020
Elevated intracellular cyclic-di-GMP level in Shewanella oneidensis increases expression of c-type cytochromes.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Biofilm engineering through c-di-GMP tuning boosts bioleaching efficiency and arsenic tolerance inApplied and environmental microbiology · 2026Article
- Impact and applications of cyclic di-GMP second messenger signaling in biotechnology and medicine.Sustainable microbiology · 2025Review
- Engineering artificial photosynthesis based on rhodopsin for CONature communications · 2023Article
- Seeing the light brings more food in the deep sea.The EMBO journal · 2023Article
- Elongated Riboflavin-Producing Shewanella oneidensis in a Hybrid Biofilm Boosts Extracellular Electron Transfer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Systematic Full-Cycle Engineering Microbial Biofilms to Boost Electricity Production inResearch (Washington, D.C.) · 2023Article
- Yin and Yang of Biofilm Formation and Cyclic di-GMP Signaling of the Gastrointestinal Pathogen Salmonella enterica Serovar Typhimurium.Journal of innate immunity · 2022Review
- Pyruvate accelerates palladium reduction by regulating catabolism and electron transfer pathway inApplied and environmental microbiology · 2021Article
- Identification of a Diguanylate Cyclase That Facilitates Biofilm Formation on Electrodes by Shewanella oneidensis MR-1.Applied and environmental microbiology · 2021Article
- Biofilm Biology and Engineering ofFrontiers in bioengineering and biotechnology · 2021Review
- Construction of an Acetate Metabolic Pathway to Enhance Electron Generation of EngineeredFrontiers in bioengineering and biotechnology · 2021Article
- Elevated intracellular cyclic-di-GMP level in Shewanella oneidensis increases expression of c-type cytochromes.Microbial biotechnology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrochemically active biofilms are capable of exchanging electrons with solid electron acceptors and have many energy and environmental applications such as bioelectricity generation and environmental remediation. The performance of electrochemically active biofilms is usually dependent on c-type cytochromes, while biofilm development is controlled by a signal cascade mediated by the intracellular secondary messenger bis-(3'-5') cyclic dimeric guanosine monophosphate (c-di-GMP). However, it is unclear whether there are any links between the c-di-GMP regulatory system and the expression of c-type cytochromes. In this study, we constructed a S. oneidensis MR-1 strain with a higher cytoplasmic c-di-GMP level by constitutively expressing a c-di-GMP synthase and it exhibited expected c-di-GMP-influenced traits, such as lowered motility and increased biofilm formation. Compared to MR-1 wild-type strain, the high c-di-GMP strain had a higher Fe(III) reduction rate (21.58 vs 11.88 pM of Fe(III)/h cell) and greater expression of genes that code for the proteins involved in the Mtr pathway, including CymA, MtrA, MtrB, MtrC and OmcA. Furthermore, single-cell Raman microspectroscopy (SCRM) revealed a great increase of c-type cytochromes in the high c-di-GMP strain as compared to MR-1 wild-type strain. Our results reveal for the first time that the c-di-GMP regulation system indirectly or directly positively regulates the expression of cytochromes involved in the extracellular electron transport (EET) in S. oneidensis, which would help to understand the regulatory mechanism of c-di-GMP on electricity production in bacteria.
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