Evidence map›Paper›PMID 32728517›Full record

Article3 Biotech2020

An affordable plasmid miniprep suitable for proficient microinjection in

Hyun-Min Kim, Sisi Tian, Shouyue Wang

Abstract read
In one paragraph

Article in 3 Biotech, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. A Decade of CRISPR-Cas Gnome Editing inInternational journal of molecular sciences · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hyun-Min KimSchool of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072 China.ORCID 0000-0003-4610-3606
Sisi TianSchool of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072 China.ORCID https://orcid.org/0000-0002-6914-100X
Shouyue WangSchool of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072 China.ORCID https://orcid.org/0000-0001-6878-572X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A variety of techniques, including CRISPR-Cas9 genome editing, have been developed to produce genetically modified cell lines and animal models. In many cases, the success of the genome-editing techniques is dependent on the quality of the introduced DNA. However, the preparation of high-quality plasmids required for small-scale microinjection has not been explored. Here, we compared various types of plasmid preparation methods for their microinjection proficiency and developed an efficient and affordable plasmid mini preparation method suitable for

Indexed as

CRISPR-Cas9Genome editingMicroinjectionPlasmid miniprep

Identifiers

PMID32728517
PMCPMC7376781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.