ArticleScientific reports2020
Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- Timelapse-based 3D reconstruction of blastocysts reveals 3D morphologies of human blastocysts.NPJ digital medicine · 2025Article
- Making sense of blobs, whorls, and shades: methods for label-free, inverse imaging in bright-field optical microscopy.Biophysical reviews · 2025Review
- Blind deconvolution in autocorrelation inversion for multiview light-sheet microscopy.Microscopy research and technique · 2022Article
- Optical sectioning of unlabeled samples using bright-field microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- High resolution optical projection tomography platform for multispectral imaging of the mouse gut.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy.
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Registered trials
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