ArticleScientific reports2020
Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio).
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
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- Evaluation of eef1a1l1 expression stability and promoter-driven reporter activity during zebrafish development.microPublication biology · 2026Article
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- Disrupted diencephalon development and neuropeptidergic pathways in zebrafish with autism-risk mutations.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Natterin bridges IFN-φ1 and non-canonical inflammasome pathways viaFrontiers in cellular and infection microbiology · 2025Article
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- Codon optimality influences homeostatic gene expression in zebrafish.G3 (Bethesda, Md.) · 2024Article
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- Parkinson's disease model in zebrafish using intraperitoneal MPTP injection.Frontiers in neuroscience · 2023Article
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- Chestnut Shell Tannins: Effects on Intestinal Inflammation and Dysbiosis in Zebrafish.Animals : an open access journal from MDPI · 2021Article
- Gnrh2 maintains reproduction in fasting zebrafish through dynamic neuronal projection changes and regulation of gonadotropin synthesis, oogenesis, and reproductive behaviors.Scientific reports · 2021Article
- Growth Hormone Overexpression Induces Hyperphagia and Intestinal Morphophysiological Adaptations to Improve Nutrient Uptake in Zebrafish.Frontiers in physiology · 2021Article
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12 authors.
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Abstract
Reference genes (RGs) must have a stable expression in tissues in all experimental conditions to normalize real-time quantitative reverse transcription PCR (qRT-PCR) data. F0104 is a highly studied lineage of zebrafish developed to overexpress the growth hormone (GH). It is assumed that the transgenic process may influence the expression levels of commonly used RGs. The objective of the present study was to make a comprehensive analysis of stability of canditade RGs actb1, actb2, b2m, eif2s2, eef1a1, gapdh, rplp2, rpl7, rpl13α, tuba1, and rps18, in gh-transgenic and non-transgenic zebrafish. Liver, brain, intestine and muscle samples from both groups had qRT-PCR results analyzed by dCt, geNorm, NormFinder, BestKeeper, and RefFinder softwares. Consensus analyses among software concluded that rpl13α, rpl7, and eef1a1 are the most stable genes for zebrafish, considering the studied groups and tissues. Gapdh, rps18, and tuba1 suffered variations in stability among different tissues of both groups, and so, they were listed as the genes with lowest stability. Results from an average pairwise variations test indicated that the use of two RGs would generate reliable results for gene expression analysis in the studied tissues. We conclude that genes that are commonly used in mammals for qRT-PCR assays have low stability in both non-transgenic and gh-transgenic zebrafish reinforcing the importance of using species-specific RGs.
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