ArticleScientific reports2020
Gene products and processes contributing to lanthanide homeostasis and methanol metabolism in Methylorubrum extorquens AM1.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Marine microbiomes and their expanding role in biotechnological potential: a systematic review.Archives of microbiology · 2026Pooled it
- Source-Independent Enrichment of Light Lanthanides: Microbial Mobilization, Selective Uptake, and Intracellular Storage.Environmental microbiology · 2026Article
- Synthesis of two methylolanthanin derivatives and the investigation of their lanthanide and iron binding capabilities.Dalton transactions (Cambridge, England : 2003) · 2026Article
- CRISPRi-based functional genomic screening identifies genes essential for CHScience advances · 2026Article
- Beneath the surface: expanding the known repertoire of methylotrophic metabolism.Applied and environmental microbiology · 2026Article
- Functional organization and regulatory logic of theApplied and environmental microbiology · 2026Review
- Urban Biomining of Rare Earth Elements: Current Status and Future Opportunities.ACS environmental Au · 2026Review
- Response and adaptation of verrucomicrobial methanotrophs to heat and acidity.Archives of microbiology · 2025Article
- XoxF and the Calvin-Benson cycle mediate lanthanide-dependent growth on methanol inApplied and environmental microbiology · 2025Article
- Diel cycle of lanthanide-dependent methylotrophy by TMED127/Methylaequorales bacteria in oligotrophic surface seawater.Applied and environmental microbiology · 2025Article
- Diversity and distribution of the lanthanome in aerobic methane-oxidising bacteria.Environmental microbiome · 2025Article
- Lanthanide-dependent isolation of phyllosphere methylotrophs selects for a phylogenetically conserved but metabolically diverse community.bioRxiv : the preprint server for biology · 2025Article
- Recent advances in metallophore research uncover functions in quorum sensing, antimicrobial activity, and lanthanide acquisition.Current opinion in chemical biology · 2025Review
- Methylobacterium extorquens PA1 utilizes multiple strategies to maintain formaldehyde homeostasis during methylotrophic growth.PLoS genetics · 2025Article
- Emerging role of rare earth elements in biomolecular functions.The ISME journal · 2025Review
- Modulating metal-centered dimerization of a lanthanide chaperone protein for separation of light lanthanides.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Electron microscopy evidence of gadolinium toxicity being mediated through cytoplasmic membrane dysregulation.Metallomics : integrated biometal science · 2024Article
- Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Rare earth elements in biology: From biochemical curiosity to solutions for extractive industries.Microbial biotechnology · 2024Review
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
Abstract
Lanthanide elements have been recently recognized as "new life metals" yet much remains unknown regarding lanthanide acquisition and homeostasis. In Methylorubrum extorquens AM1, the periplasmic lanthanide-dependent methanol dehydrogenase XoxF1 produces formaldehyde, which is lethal if allowed to accumulate. This property enabled a transposon mutagenesis study and growth studies to confirm novel gene products required for XoxF1 function. The identified genes encode an MxaD homolog, an ABC-type transporter, an aminopeptidase, a putative homospermidine synthase, and two genes of unknown function annotated as orf6 and orf7. Lanthanide transport and trafficking genes were also identified. Growth and lanthanide uptake were measured using strains lacking individual lanthanide transport cluster genes, and transmission electron microscopy was used to visualize lanthanide localization. We corroborated previous reports that a TonB-ABC transport system is required for lanthanide incorporation to the cytoplasm. However, cells were able to acclimate over time and bypass the requirement for the TonB outer membrane transporter to allow expression of xoxF1 and growth. Transcriptional reporter fusions show that excess lanthanides repress the gene encoding the TonB-receptor. Using growth studies along with energy dispersive X-ray spectroscopy and transmission electron microscopy, we demonstrate that lanthanides are stored as cytoplasmic inclusions that resemble polyphosphate granules.
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Registered trials
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