ArticleScientific reports2020
Unveiling functional heterogeneity in breast cancer multicellular tumor spheroids through single-cell RNA-seq.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Programmed cell death and metastatic evolution in breast cancer: the role of anoikis, necroptosis, and ferroptosis.Apoptosis : an international journal on programmed cell death · 2026Review
- Radiobioconjugate of Kadcyla with Radioactive Gold Nanoparticles for Targeted Therapy of HER2-Overexpressing Cancers.Molecular pharmaceutics · 2025Article
- Advancing cancer research through 3D cell culture models.EXCLI journal · 2025Review
- Intratumoral Heterogeneity and Metabolic Cross-Feeding in a Three-Dimensional Breast Cancer Culture: An In Silico Perspective.International journal of molecular sciences · 2024Article
- Single-Cell RNA-Sequencing: Opening New Horizons for Breast Cancer Research.International journal of molecular sciences · 2024Review
- Article
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- Genomic analysis of plasma circulating tumor DNA in patients with heavily pretreated HER2 + metastatic breast cancer.Scientific reports · 2023Article
- MUG CCArly: A Novel Autologous 3D Cholangiocarcinoma Model Presents an Increased Angiogenic Potential.Cancers · 2023Article
- How to build a tumor: An industry perspective.Drug discovery today · 2022Review
- Estimating intraclonal heterogeneity and subpopulation changes from bulk expression profiles in CMap.Life science alliance · 2022Article
- Review
- Longitudinal Shifts of Solid Tumor and Liquid Biopsy Sequencing Concordance in Metastatic Breast Cancer.JCO precision oncology · 2022Article
- Ultrasound Triggers Hypericin Activation Leading to Multifaceted Anticancer Activity.Pharmaceutics · 2022Article
- Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines.Cancer & metabolism · 2022Article
- Effects of collagen membranes and bone substitute differ in periodontal ligament cell microtissues and monolayers.Journal of periodontology · 2022Article
- Development of a Multicellular 3D Tumor Model to Study Cellular Heterogeneity and Plasticity in NSCLC Tumor Microenvironment.Frontiers in oncology · 2022Article
- Single cell organization and cell cycle characterization of DNA stained multicellular tumor spheroids.Scientific reports · 2021Article
- Activity of trastuzumab emtansine (T-DM1) in 3D cell culture.Breast cancer research and treatment · 2021Article
- Advances in removing mass transport limitations for more physiologically relevantBiophysics reviews · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterogeneity is an intrinsic characteristic of cancer. Even in isogenic tumors, cell populations exhibit differential cellular programs that overall supply malignancy and decrease treatment efficiency. In this study, we investigated the functional relationship among cell subtypes and how this interdependency can promote tumor development in a cancer cell line. To do so, we performed single-cell RNA-seq of MCF7 Multicellular Tumor Spheroids as a tumor model. Analysis of single-cell transcriptomes at two-time points of the spheroid growth, allowed us to dissect their functional relationship. As a result, three major robust cellular clusters, with a non-redundant complementary composition, were found. Meanwhile, one cluster promotes proliferation, others mainly activate mechanisms to invade other tissues and serve as a reservoir population conserved over time. Our results provide evidence to see cancer as a systemic unit that has cell populations with task stratification with the ultimate goal of preserving the hallmarks in tumors.
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