ArticleScientific reports2020
Novel microwire-based biosensor probe for simultaneous real-time measurement of glutamate and GABA dynamics in vitro and in vivo.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Electrothermal Synthesis of Cell-Imprinted Polymer Coatings on Metallic Microwires for Bacterial Capture.Sensors (Basel, Switzerland) · 2026Article
- Creation of an Engineered Oxygen-Insensitive L-Glutamate Oxidase for the Application of Electrochemical L-Glutamate Sensors.International journal of molecular sciences · 2026Article
- Aberrant neuronal firing: a paracrine route to glioblastoma expansion.Cell communication and signaling : CCS · 2025Article
- Enzyme-modified microelectrodes for measurement of glutamate: Characterization and applications.Electroanalysis · 2025Article
- Enhancing the sensitivity of gamma-aminobutyric acid and glutamate biosensors by electrochemically roughening platinum microelectrodes.Frontiers in neuroscience · 2025Article
- Improving Sensitivity and Longevity of In Vivo Glutamate Sensors with Electrodeposited NanoPt.ACS applied materials & interfaces · 2024Article
- Bidirectional Neuronal Control of Epileptiform Activity by Repetitive Transcranial Focused Ultrasound Stimulations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Editors' Choice-Review-The Future of Carbon-Based Neurochemical Sensing: A Critical Perspective.ECS sensors plus · 2023Review
- An Updated Review on Electrochemical Nanobiosensors for Neurotransmitter Detection.Biosensors · 2023Review
- The Partial MACS chemical neuroscience · 2023Article
- Streamlining the interface between electronics and neural systems for bidirectional electrochemical communication.Chemical science · 2023Review
- Microglia and astrocytes underlie neuroinflammation and synaptic susceptibility in autism spectrum disorder.Frontiers in neuroscience · 2023Review
- Towards Multiplexed and Multimodal Biosensor Platforms in Real-Time Monitoring of Metabolic Disorders.Sensors (Basel, Switzerland) · 2022Review
- Brain-Implantable Multifunctional Probe for Simultaneous Detection of Glutamate and GABA Neurotransmitters: Optimization and In Vivo Studies.Micromachines · 2022Article
- Rapid Detection of Anti-SARS-CoV-2 Antibodies with a Screen-Printed Electrode Modified with a Spike Glycoprotein Epitope.Biosensors · 2022Article
- Perspective-Electrochemical Sensors for Neurotransmitters and Psychiatrics: Steps toward Physiological Mental Health Monitoring.Journal of the Electrochemical Society · 2022Article
- A Pencil-Lead Immunosensor for the Rapid Electrochemical Measurement of Anti-Diphtheria Toxin Antibodies.Biosensors · 2021Article
- Fabrication of Highly Sensitive Pt-black Electrochemical Sensors for GABA Detection.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2021Article
- Brain-Implantable Multifunctional Probe for Simultaneous Detection of Glutamate and GABA Neurotransmitters.Sensors and actuators. B, Chemical · 2021Article
- Recent Advances in In Vivo Neurochemical Monitoring.Micromachines · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutamate (GLU) and γ-aminobutyric acid (GABA) are the major excitatory (E) and inhibitory (I) neurotransmitters in the brain, respectively. Dysregulation of the E/I ratio is associated with numerous neurological disorders. Enzyme-based microelectrode array biosensors present the potential for improved biocompatibility, localized sample volumes, and much faster sampling rates over existing measurement methods. However, enzymes degrade over time. To overcome the time limitation of permanently implanted microbiosensors, we created a microwire-based biosensor that can be periodically inserted into a permanently implanted cannula. Biosensor coatings were based on our previously developed GLU and reagent-free GABA shank-type biosensor. In addition, the microwire biosensors were in the same geometric plane for the improved acquisition of signals in planar tissue including rodent brain slices, cultured cells, and brain regions with laminar structure. We measured real-time dynamics of GLU and GABA in rat hippocampal slices and observed a significant, nonlinear shift in the E/I ratio from excitatory to inhibitory dominance as electrical stimulation frequency increased from 10 to 140 Hz, suggesting that GABA release is a component of a homeostatic mechanism in the hippocampus to prevent excitotoxic damage. Additionally, we recorded from a freely moving rat over fourteen weeks, inserting fresh biosensors each time, thus demonstrating that the microwire biosensor overcomes the time limitation of permanently implanted biosensors and that the biosensors detect relevant changes in GLU and GABA levels that are consistent with various behaviors.
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