ArticleBMC plant biology2020
De novo assembly and Transcriptome characterization of an endemic species of Vietnam, Panax vietnamensis Ha et Grushv., including the development of EST-SSR markers for population genetics.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Leveraging next-generation sequencing technology for the study of ginsenosides biosynthesis and exploring DNA markers in the endemic species Panax vietnamensis.Molecular biology reports · 2024Pooled it
- Comparative Analysis of the Ginsenosides inMolecules (Basel, Switzerland) · 2026Article
- Transcriptome characterisation, SSR marker development and genetic diversity analysis of the endangered speciesBiodiversity data journal · 2026Article
- Transcriptome characterization and population genetics ofBiodiversity data journal · 2026Article
- De Novo Transcriptome Assembly ofPlants (Basel, Switzerland) · 2024Article
- Transcriptome sequencing of the endangered land snail Karaftohelix adamsi from the Island Ulleung: De novo assembly, annotation, valuation of fitness genes and SSR markers.Genes & genomics · 2024Article
- Suspension cell cultures ofFrontiers in plant science · 2024Article
- Phylogeographic analysis reveals extensive genetic variation of native grassFrontiers in plant science · 2024Article
- Population genetics analysis ofBiodiversity data journal · 2024Article
- Genetic variation and structure of endemic and endangered wild celery (Frontiers in plant science · 2024Article
- Development of unigene-derived SSR markers from RNA-seq data of Uraria lagopodioides (Fabaceae) and their application in the genus Uraria Desv. (Fabaceae).BMC plant biology · 2023Article
- Article
- Molecular Characterization ofGenes · 2022Article
- Article
- De novo transcriptome assembly, gene annotation, and EST-SSR marker development of an important medicinal and edible crop, Amomum tsaoko (Zingiberaceae).BMC plant biology · 2022Article
- Upcoming progress of transcriptomics studies on plants: An overview.Frontiers in plant science · 2022Review
- Target Capture Reveals the Complex Origin of Vietnamese Ginseng.Frontiers in plant science · 2022Article
- De novo whole-genome assembly and discovery of genes involved in triterpenoid saponin biosynthesis of Vietnamese ginseng (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Research Progress and Future Development Trends in Medicinal Plant Transcriptomics.Frontiers in plant science · 2021Review
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6 authors.
Funding
Abstract
backgroundUnderstanding the genetic diversity in endangered species that occur inforest remnants is necessary to establish efficient strategies for the species conservation, restoration and management. Panax vietnamensis Ha et Grushv. is medicinally important, endemic and endangered species of Vietnam. However, genetic diversity and structure of population are unknown due to lack of efficient molecular markers.
resultsIn this study, we employed Illumina HiSeq™ 4000 sequencing to analyze the transcriptomes of P. vietnamensis (roots, leaves and stems). Raw reads total of 23,741,783 was obtained and then assembled, from which the generated unigenes were 89,271 (average length = 598.3191 nt). The 31,686 unigenes were annotated in different databases i.e. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, Nucleotide Collection (NR/NT) and Swiss-Prot for functional annotation. Further, 11,343 EST-SSRs were detected. From 7774 primer pairs, 101 were selected for polymorphism validation, in which; 20 primer pairs were successfully amplified to DNA fragments and significant amounts of polymorphism was observed within population. The nine polymorphic microsatellite loci were used for population structure and diversity analyses. The obtained results revealed high levels of genetic diversity in populations, the average observed and expected heterozygosity were H
conclusionOur study will assist conservators in future conservation management, breeding, production and habitats restoration of the species.
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